Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis.

Animals Apoptosis Biomarkers, Tumor / genetics Cell Movement Cell Proliferation Disease Progression Gene Expression Regulation, Neoplastic Homeodomain Proteins / genetics Humans Laryngeal Neoplasms / genetics Male Mice Mice, Inbred BALB C Mice, Nude MicroRNAs / genetics Microfilament Proteins / genetics Neoplasm Invasiveness Prognosis Proto-Oncogene Proteins / genetics RNA, Circular / genetics Squamous Cell Carcinoma of Head and Neck / genetics Tumor Cells, Cultured Xenograft Model Antitumor Assays
Epithelial–mesenchymal transition Laryngeal squamous cell carcinoma Let-7c-5p Metastasis PBX3 circCORO1C

Journal

Molecular cancer
ISSN: 1476-4598
Titre abrégé: Mol Cancer
Pays: England
ID NLM: 101147698

Informations de publication

Date de publication:
02 06 2020
Historique:
received: 20 01 2020
accepted: 11 05 2020
entrez: 4 6 2020
pubmed: 4 6 2020
medline: 18 2 2021
Statut: epublish

Résumé

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck. LSCC patients have seriously impaired vocal, respiratory, and swallowing functions with poor prognosis. Circular RNA (circRNA) has attracted great attention in cancer research. However, the expression patterns and roles of circRNAs in LSCC remain largely unknown. RNA sequencing was performed on 57 pairs of LSCC and matched adjacent normal mucosa tissues to construct circRNA, miRNA, and mRNA expression profiles. RT-PCR, qPCR, Sanger sequencing, and FISH were undertaken to study the expression, localization, and clinical significance of circCORO1C in LSCC tissues and cells. The functions of circCORO1C in LSCC were investigated by RNAi-mediated knockdown, proliferation analysis, EdU staining, colony formation assay, Transwell assay, and apoptosis analysis. The regulatory mechanisms among circCORO1C, let-7c-5p, and PBX3 were investigated by luciferase assay, RNA immunoprecipitation, western blotting, and immunohistochemistry. circCORO1C was highly expressed in LSCC tissues and cells, and this high expression was closely associated with the malignant progression and poor prognosis of LSCC. Knockdown of circCORO1C inhibited the proliferation, migration, invasion, and in vivo tumorigenesis of LSCC cells. Mechanistic studies revealed that circCORO1C competitively bound to let-7c-5p and prevented it from decreasing the level of PBX3, which promoted the epithelial-mesenchymal transition and finally facilitated the malignant progression of LSCC. circCORO1C has an oncogenic role in LSCC progression and may serve as a novel target for LSCC therapy. circCORO1C expression has the potential to serve as a novel diagnostic and prognostic biomarker for LSCC detection.

Sections du résumé

BACKGROUND
Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck. LSCC patients have seriously impaired vocal, respiratory, and swallowing functions with poor prognosis. Circular RNA (circRNA) has attracted great attention in cancer research. However, the expression patterns and roles of circRNAs in LSCC remain largely unknown.
METHODS
RNA sequencing was performed on 57 pairs of LSCC and matched adjacent normal mucosa tissues to construct circRNA, miRNA, and mRNA expression profiles. RT-PCR, qPCR, Sanger sequencing, and FISH were undertaken to study the expression, localization, and clinical significance of circCORO1C in LSCC tissues and cells. The functions of circCORO1C in LSCC were investigated by RNAi-mediated knockdown, proliferation analysis, EdU staining, colony formation assay, Transwell assay, and apoptosis analysis. The regulatory mechanisms among circCORO1C, let-7c-5p, and PBX3 were investigated by luciferase assay, RNA immunoprecipitation, western blotting, and immunohistochemistry.
RESULTS
circCORO1C was highly expressed in LSCC tissues and cells, and this high expression was closely associated with the malignant progression and poor prognosis of LSCC. Knockdown of circCORO1C inhibited the proliferation, migration, invasion, and in vivo tumorigenesis of LSCC cells. Mechanistic studies revealed that circCORO1C competitively bound to let-7c-5p and prevented it from decreasing the level of PBX3, which promoted the epithelial-mesenchymal transition and finally facilitated the malignant progression of LSCC.
CONCLUSIONS
circCORO1C has an oncogenic role in LSCC progression and may serve as a novel target for LSCC therapy. circCORO1C expression has the potential to serve as a novel diagnostic and prognostic biomarker for LSCC detection.

Identifiants

DOI: 10.1186/s12943-020-01215-4 PMID: 32487167 PMC: PMC7265647
pubmed: 32487167
doi: 10.1186/s12943-020-01215-4
pii: 10.1186/s12943-020-01215-4
pmc: PMC7265647
doi:

Substances chimiques

Biomarkers, Tumor 0
Homeodomain Proteins 0
MicroRNAs 0
Microfilament Proteins 0
Proto-Oncogene Proteins 0
RNA, Circular 0
mirnlet7 microRNA, human 0
coronin proteins 145420-64-0
proto-oncogene protein Pbx3 146150-81-4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

99

Commentaires et corrections

Type : ErratumIn
Type : ErratumIn

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Auteurs

Yongyan Wu (Y)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Biochemistry & Molecular Biology, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Yuliang Zhang (Y)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Xiwang Zheng (X)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Fengsheng Dai (F)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Yan Lu (Y)

Department of Otolaryngology Head & Neck Surgery, The First Hospital, Jinzhou Medical University, Jinzhou, 121001, Liaoning, People's Republic of China.

Li Dai (L)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Min Niu (M)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Huina Guo (H)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Wenqi Li (W)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Xuting Xue (X)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Yunfeng Bo (Y)

Department of Pathology, Shanxi Cancer Hospital, Shanxi Medical University, Taiyuan, 030013, Shanxi, People's Republic of China.

Yujia Guo (Y)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Jiangbo Qin (J)

Department of Otolaryngology Head & Neck Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, 046000, Shanxi, People's Republic of China.

Yixiao Qin (Y)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Hongliang Liu (H)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Cell Biology and Genetics, Basic Medical School of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Yu Zhang (Y)

Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.
Department of Physiology, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Tao Yang (T)

Department of Biochemistry & Molecular Biology, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Li Li (L)

Department of Cell Biology and Genetics, Basic Medical School of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China.

Linshi Zhang (L)

Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, People's Republic of China.

Rui Hou (R)

Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for Medical Research, the University of Western Australia, PO Box 7214, 6 Verdun Street, Nedlands, Perth, Western Australia, 6009, Australia.

Shuxin Wen (S)

General Hospital, Shenzhen University, Shenzhen, 518055, Guangdong, People's Republic of China.

Changming An (C)

Department of Head and Neck Surgery, Cancer Hospital, National Cancer Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100021, People's Republic of China. anchangming@cicams.ac.cn.

Huizheng Li (H)

Department of Otolaryngology Head & Neck Surgery, Dalian Municipal Friendship Hospital, Dalian Medical University, Dalian, 116100, Liaoning, People's Republic of China. huizhengli2004@163.com.

Wei Xu (W)

Shandong Provincial ENT Hospital Affiliated to Shandong University, Jinan, 250022, Shandong, People's Republic of China. xuwhns@126.com.
Shandong Provincial Institute of Otolaryngology, Jinan, 250022, Shandong, People's Republic of China. xuwhns@126.com.
Key Laboratory of Otolaryngology, Ministry of Health, Shandong University, Jinan, 250022, Shandong, People's Republic of China. xuwhns@126.com.

Wei Gao (W)

Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. gaoweisxent@sxent.org.
Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. gaoweisxent@sxent.org.
Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. gaoweisxent@sxent.org.
Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. gaoweisxent@sxent.org.
Department of Cell Biology and Genetics, Basic Medical School of Shanxi Medical University, Taiyuan, 030001, Shanxi, People's Republic of China. gaoweisxent@sxent.org.
ORCID: 0000-0001-7836-2851

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Phénomènes physiologiques › Mouvement cellulaire › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Caractéristiques de la maladie › Évolution de la maladie › Circular RNA circCORO1C promotes laryngeal...
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questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée BALB C › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souches mutantes de souris › Souris nude › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Circular RNA circCORO1C promotes laryngeal...
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questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN circulaire › Circular RNA circCORO1C promotes laryngeal...
questionsmedicales.fr › Tumeurs › Tumeurs par siège › Tumeurs de la tête et du cou › Carcinome épidermoïde de la tête et du cou › Circular RNA circCORO1C promotes laryngeal...
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