Calcium enhances polyplex-mediated transfection efficiency of plasmid DNA in Jurkat cells.
Jurkat
T-cells
calcium chloride
divalent cation
gene delivery
gene therapy
lipoplex
poly(ethylene imine)
polyplex
transfection
Journal
Drug delivery
ISSN: 1521-0464
Titre abrégé: Drug Deliv
Pays: England
ID NLM: 9417471
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
30
3
2021
Statut:
ppublish
Résumé
Jurkat, an immortalized cell line derived from human leukemic T lymphocytes, has been employed as an excellent surrogate model of human primary T-cells for the advancement of T-cell biology and their applications in medicine. However, presumably due to its T-cell origin, Jurkat cells are very difficult to transfect. Thus, for the genetic modification of Jurkat cells, expensive and time-consuming viral vectors are normally required. Despite many previous efforts, non-viral vectors have not yet overcome the hurdles of low transfection efficiency and/or high toxicity in transfection of Jurkat cells. Here, we report that a simple addition of calcium ions (Ca
Identifiants
pubmed: 32489110
doi: 10.1080/10717544.2020.1770371
pmc: PMC8216448
doi:
Substances chimiques
Cations, Divalent
0
Imines
0
Polyethylenes
0
poly(ethylene imine)
0
Calcium Chloride
M4I0D6VV5M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
805-815Références
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