Viability evaluation of layered cell sheets after ultraviolet light irradiation of 222 nm.
3D, 3-dementional
CPDs, cyclobutane-pyrimidine dimers
Cell sheet
Cellular viability
H2O2, hydrogen peroxide
Kr–Cl, krypton-chloride
MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide
PBS, phosphate-buffered saline
SD, standard deviation
Sterilization
UV
Journal
Regenerative therapy
ISSN: 2352-3204
Titre abrégé: Regen Ther
Pays: Netherlands
ID NLM: 101709085
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
04
03
2020
revised:
17
03
2020
accepted:
04
04
2020
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
4
6
2020
Statut:
epublish
Résumé
The objective of this study was to evaluate the cell viability of layered cell sheets, irradiated with 222 nm UV light. UV transmittance of 222 nm and 254 nm was evaluated when the cell sheets of NCTC Clone 929 cells were irradiated UV light. Cell viability was evaluated after irradiation of 222 nm using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. Following irradiation of two layered cell sheets at 500 mJ/cm The UV transmittance of 222 nm was 10 times less than that of 254 nm. A MTT assay revealed that cells of cell sheets irradiated at 222 nm was less damaged than those at 254 nm, when irradiated at 5 mJ/cm It is concluded that 222 nm irradiation is biologically safe for cell viability.
Identifiants
pubmed: 32490060
doi: 10.1016/j.reth.2020.04.002
pii: S2352-3204(20)30018-3
pmc: PMC7260610
doi:
Types de publication
Journal Article
Langues
eng
Pagination
344-351Informations de copyright
© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
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