Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of
Bioluminescence microscopy
Drosophila pupa
Engrailed
Firefly luciferase
Gal4-UAS system
Journal
Biochemistry and biophysics reports
ISSN: 2405-5808
Titre abrégé: Biochem Biophys Rep
Pays: Netherlands
ID NLM: 101660999
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
16
01
2020
revised:
11
04
2020
accepted:
18
05
2020
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
4
6
2020
Statut:
epublish
Résumé
Bioluminescence microscopy is an area attracting considerable interest in the field of cell biology because it offers several advantages over fluorescence microscopy, including no requirement for excitation light and being phototoxicity free. This method requires brighter luciferase for imaging; however, suitable genetic resource material for this purpose is not available at present. To achieve brighter bioluminescence microscopy, we developed a new firefly luciferase. Using the brighter luciferase, a reporter strain of
Identifiants
pubmed: 32490216
doi: 10.1016/j.bbrep.2020.100771
pii: S2405-5808(20)30080-7
pii: 100771
pmc: PMC7262549
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100771Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors K. Ogoh, R. Akiyoshi, and H. Suzuki are employees of Olympus Corporation (Tokyo, Japan) and are inventors on patent applications US9523082, JP6032861, JP6006070, JP5980608, JP5896624, and JP5860651 submitted by Olympus, Nimura Genetic Solutions (Tokyo, Japan) and Perak State Development Corporation (Perak, Malaysia) that covers the modified luciferase genes.
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