Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons.
Ca2+
HEPES
iPSC
lysosomal disease
lysosome
neuron
zwitterionic buffer
Journal
AMRC open research
ISSN: 2517-6900
Titre abrégé: AMRC Open Res
Pays: England
ID NLM: 101755131
Informations de publication
Date de publication:
18 May 2020
18 May 2020
Historique:
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
4
6
2020
Statut:
ppublish
Résumé
Good's buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced pluripotent stem cell-derived neurons, this study explores the effect of zwitterionic buffers, specifically HEPES, on lysosomal volume and Ca
Identifiants
pubmed: 32490352
doi: 10.12688/amrcopenres.12903.1
pmc: PMC7266671
mid: EMS86491
doi:
Banques de données
figshare
['10.6084/m9.figshare.12218441.v1']
Types de publication
Journal Article
Langues
eng
Pagination
21Subventions
Organisme : Medical Research Council
ID : MR/L023784/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L023784/2
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P007651/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
No competing interests were disclosed.
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