A single-synapse resolution survey of PSD95-positive synapses in twenty human brain regions.
automated image analysis
human post-mortem brain tissue
postsynaptic density protein 95
regional diversity
synaptome atlas
synaptome mapping
Journal
The European journal of neuroscience
ISSN: 1460-9568
Titre abrégé: Eur J Neurosci
Pays: France
ID NLM: 8918110
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
20
05
2020
received:
18
01
2020
accepted:
21
05
2020
pubmed:
4
6
2020
medline:
4
11
2021
entrez:
4
6
2020
Statut:
ppublish
Résumé
Mapping the molecular composition of individual excitatory synapses across the mouse brain reveals high synapse diversity with each brain region showing a distinct composition of synapse types. As a first step towards systematic mapping of synapse diversity across the human brain, we have labelled and imaged synapses expressing the excitatory synapse protein PSD95 in twenty human brain regions, including 13 neocortical, two subcortical, one hippocampal, one cerebellar and three brainstem regions, in four phenotypically normal individuals. We quantified the number, size and intensity of individual synaptic puncta and compared their regional distributions. We found that each region showed a distinct signature of synaptic puncta parameters. Comparison of brain regions showed that cortical and hippocampal structures are similar, and distinct from those of cerebellum and brainstem. Comparison of synapse parameters from human and mouse brain revealed conservation of parameters, hierarchical organization of brain regions and network architecture. This work illustrates the feasibility of generating a systematic single-synapse resolution atlas of the human brain, a potentially significant resource in studies of brain health and disease.
Substances chimiques
Disks Large Homolog 4 Protein
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6864-6881Subventions
Organisme : Medical Research Council
ID : MR/L016400/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 202932
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0802238
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1000419
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. European Journal of Neuroscience published by Federation of European Neuroscience Societies and John Wiley & Sons Ltd.
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