Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
26
05
2019
accepted:
28
01
2020
entrez:
5
6
2020
pubmed:
5
6
2020
medline:
5
6
2020
Statut:
epublish
Résumé
The process by which a zygote develops from a single cell into a multicellular organism is poorly understood. Advances are hindered by detection specificity and sensitivity limitations of single-cell protein tools and by challenges in integrating multimodal data. We introduce an open microfluidic tool expressly designed for same-cell phenotypic, protein, and mRNA profiling. We examine difficult-to-study-yet critically important-murine preimplantation embryo stages. In blastomeres dissociated from less well-studied two-cell embryos, we observe no significant GADD45a protein expression heterogeneity, apparent at the four-cell stage. In oocytes, we detect differences in full-length versus truncated DICER-1 mRNA and protein, which are insignificant by the two-cell stage. Single-embryo analyses reveal intraembryonic heterogeneity, differences between embryos of the same fertilization event and between donors, and reductions in the burden of animal sacrifice. Open microfluidic design integrates with existing workflows and opens new avenues for assessing the cellular-to-molecular heterogeneity inherent to preimplantation embryo development.
Identifiants
pubmed: 32494630
doi: 10.1126/sciadv.aay1751
pii: aay1751
pmc: PMC7176412
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eaay1751Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008155
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA203018
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114414
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA139067
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD088885
Pays : United States
Organisme : NCI NIH HHS
ID : F32 CA192636
Pays : United States
Organisme : NICHD NIH HHS
ID : K99 HD096108
Pays : United States
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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