The +TIP Navigator-1 is an actin-microtubule crosslinker that regulates axonal growth cone motility.
Actin Cytoskeleton
/ metabolism
Actins
/ metabolism
Animals
Axon Guidance
/ physiology
Axons
/ metabolism
Cell Line
Cell Movement
/ physiology
Female
Growth Cones
/ metabolism
HEK293 Cells
Humans
Mice
Microtubule-Associated Proteins
/ metabolism
Microtubules
/ metabolism
Netrin-1
/ metabolism
Protein Binding
/ physiology
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 09 2020
07 09 2020
Historique:
received:
27
05
2019
revised:
03
04
2020
accepted:
08
05
2020
entrez:
5
6
2020
pubmed:
5
6
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
Microtubule (MT) plus-end tracking proteins (+TIPs) are central players in the coordination between the MT and actin cytoskeletons in growth cones (GCs) during axon guidance. The +TIP Navigator-1 (NAV1) is expressed in the developing nervous system, yet its neuronal functions remain poorly elucidated. Here, we report that NAV1 controls the dynamics and motility of the axonal GCs of cortical neurons in an EB1-dependent manner and is required for axon turning toward a gradient of netrin-1. NAV1 accumulates in F-actin-rich domains of GCs and binds actin filaments in vitro. NAV1 can also bind MTs independently of EB1 in vitro and crosslinks nonpolymerizing MT plus ends to actin filaments in axonal GCs, preventing MT depolymerization in F-actin-rich areas. Together, our findings pinpoint NAV1 as a key player in the actin-MT crosstalk that promotes MT persistence at the GC periphery and regulates GC steering. Additionally, we present data assigning to NAV1 an important role in the radial migration of cortical projection neurons in vivo.
Identifiants
pubmed: 32497170
pii: 151835
doi: 10.1083/jcb.201905199
pmc: PMC7480110
pii:
doi:
Substances chimiques
Actins
0
Microtubule-Associated Proteins
0
Netrin-1
158651-98-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Sánchez-Huertas et al.
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