Peripheral Neuronopathy Associated With Ebola Virus Infection in Rhesus Macaques: A Possible Cause of Neurological Signs and Symptoms in Human Ebola Patients.
Animals
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Disease Models, Animal
Ebolavirus
Female
Ganglia
Ganglia, Spinal
/ pathology
Ganglion Cysts
/ pathology
Hemorrhagic Fever, Ebola
/ complications
Humans
Immunohistochemistry
Leukocytes, Mononuclear
Macaca mulatta
Macrophages
/ pathology
Male
Microglia
/ pathology
Necrosis
Nerve Degeneration
/ complications
Parasympathetic Nervous System
/ pathology
Peripheral Nervous System Diseases
/ complications
Sensory Receptor Cells
/ pathology
Sympathetic Nervous System
/ pathology
Ebola virus
autonomic ganglion
dorsal root ganglion
enteric plexus
parasympathetic nervous system
peripheral nervous system
somatic ganglion
sympathetic nervous system
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
13 10 2020
13 10 2020
Historique:
received:
26
02
2020
accepted:
27
05
2020
pubmed:
5
6
2020
medline:
12
3
2021
entrez:
5
6
2020
Statut:
ppublish
Résumé
Neurological signs and symptoms are the most common complications of Ebola virus disease. However, the mechanisms underlying the neurologic manifestations in Ebola patients are not known. In this study, peripheral ganglia were collected from 12 rhesus macaques that succumbed to Ebola virus (EBOV) disease from 5 to 8 days post exposure. Ganglionitis, characterized by neuronal degeneration, necrosis, and mononuclear leukocyte infiltrates, was observed in the dorsal root, autonomic, and enteric ganglia. By immunohistochemistry, RNAscope in situ hybridization, transmission electron microscopy, and confocal microscopy, we confirmed that CD68+ macrophages are the target cells for EBOV in affected ganglia. Further, we demonstrated that EBOV can induce satellite cell and neuronal apoptosis and microglial activation in infected ganglia. Our results demonstrate that EBOV can infect peripheral ganglia and results in ganglionopathy in rhesus macaques, which may contribute to the neurological signs and symptoms observed in acute and convalescent Ebola virus disease in human patients.
Identifiants
pubmed: 32498080
pii: 5851385
doi: 10.1093/infdis/jiaa304
pmc: PMC7751569
doi:
Substances chimiques
Antigens, CD
0
Antigens, Differentiation, Myelomonocytic
0
CD68 antigen, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1745-1755Subventions
Organisme : NIAID NIH HHS
ID : HHSN272200700016I
Pays : United States
Informations de copyright
Published by Oxford University Press for the Infectious Diseases Society of America 2020.
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