Human Brain Shows Recurrent Non-Canonical MicroRNA Editing Events Enriched for Seed Sequence with Possible Functional Consequence.
RNA editing
human brain
microRNA
Journal
Non-coding RNA
ISSN: 2311-553X
Titre abrégé: Noncoding RNA
Pays: Switzerland
ID NLM: 101652294
Informations de publication
Date de publication:
02 Jun 2020
02 Jun 2020
Historique:
received:
28
04
2020
revised:
25
05
2020
accepted:
27
05
2020
entrez:
6
6
2020
pubmed:
6
6
2020
medline:
6
6
2020
Statut:
epublish
Résumé
RNA editing is a post-transcriptional modification, which can provide tissue-specific functions not encoded in DNA. Adenosine-to-inosine is the predominant editing event and, along with cytosine-to-uracil changes, constitutes canonical editing. The rest is non-canonical editing. In this study, we have analysed non-canonical editing of microRNAs in the human brain. We have performed massively parallel small RNA sequencing of frontal cortex (FC) and corpus callosum (CC) pairs from nine normal individuals (post-mortem). We found 113 and 90 unique non-canonical editing events in FC and CC samples, respectively. More than 70% of events were in the miRNA seed sequence-implicating an altered set of target mRNAs and possibly resulting in a functional consequence. Up to 15% of these events were recurring and found in at least three samples, also supporting the biological relevance of such variations. Two specific sequence variations, C-to-A and G-to-U, accounted for over 80% of non-canonical miRNA editing events-and revealed preferred sequence motifs. Our study is one of the first reporting non-canonical editing in miRNAs in the human brain. Our results implicate miRNA non-canonical editing as one of the contributing factors towards transcriptomic diversity in the human brain.
Identifiants
pubmed: 32498345
pii: ncrna6020021
doi: 10.3390/ncrna6020021
pmc: PMC7345632
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Council of Scientific and Industrial Research, India
ID : BSC-0123
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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