Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water.


Journal

Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366

Informations de publication

Date de publication:
02 Jun 2020
Historique:
received: 14 05 2020
revised: 26 05 2020
accepted: 29 05 2020
entrez: 6 6 2020
pubmed: 6 6 2020
medline: 11 3 2021
Statut: epublish

Résumé

Water hardness (WH) is a useful parameter for testing household water, such as drinking, cooking, and washing water. Many countries around the world use pipeline water in their houses, but there is a need to monitor the WH because hard water has a negative impact on appliances. Currently, WH is often measured using chemical dye-based WH indicators, and these techniques require expensive equipment, and trained personnel. Therefore, a low-cost and simple measurement method has been desired. Here, we report LOTUS-W, which consists of a luciferase, Nanoluc, a yellow fluorescent protein Venus, and a Ca

Identifiants

pubmed: 32498467
pii: s20113164
doi: 10.3390/s20113164
pmc: PMC7308811
pii:
doi:

Substances chimiques

CETN3 protein, human 0
Calcium-Binding Proteins 0
Drinking Water 0
Luminescent Proteins 0
Luciferases EC 1.13.12.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Grant-in-Aid for Scientific Research on Innovative Areas" "Singularity biology", JST SENTAN KEISOKU program "Development of all in one chemiluminescence microscopeInterplay of developmental clock and extracellular environment in brain formation;
ID : No. 18H05410, No. JP16H06487

Déclaration de conflit d'intérêts

The authors have filed a domestic (Japanese) patent application for Water hardness measurement (No. 2019-006110).

Références

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Auteurs

Md Nadim Hossain (MN)

The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

Ryuichi Ishida (R)

The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.

Mitsuru Hattori (M)

The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

Tomoki Matsuda (T)

The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

Takeharu Nagai (T)

The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Japan.

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Classifications MeSH