TRACE: An Unbiased Method to Permanently Tag Transiently Activated Inputs.

innate fear mouse behavior neural circuits optogenetics viral tracing

Journal

Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935

Informations de publication

Date de publication:
2020
Historique:
received: 05 02 2020
accepted: 08 04 2020
entrez: 6 6 2020
pubmed: 6 6 2020
medline: 6 6 2020
Statut: epublish

Résumé

A fundamental interest in circuit analysis is to parse out the synaptic inputs underlying a behavioral experience. Toward this aim, we have devised an unbiased strategy that specifically labels the afferent inputs that are activated by a defined stimulus in an activity-dependent manner. We validated this strategy in four brain circuits receiving known sensory inputs. This strategy, as demonstrated here, accurately identifies these inputs.

Identifiants

pubmed: 32499680
doi: 10.3389/fncel.2020.00114
pmc: PMC7243865
doi:

Types de publication

Journal Article

Langues

eng

Pagination

114

Informations de copyright

Copyright © 2020 Krauth, Khalil, Jariwala, Mermet-Joret, Vestergaard, Capogna and Nabavi.

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Auteurs

Nathalie Krauth (N)

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.

Valentina Khalil (V)

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.

Meet Jariwala (M)

Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Center for Proteins in Memory-PROMEMO, Danish National Research Foundation, Aarhus, Denmark.

Noemie Mermet-Joret (N)

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.

Anne-Katrine Vestergaard (AK)

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.

Marco Capogna (M)

DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.
Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Center for Proteins in Memory-PROMEMO, Danish National Research Foundation, Aarhus, Denmark.

Sadegh Nabavi (S)

Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.
Center for Proteins in Memory-PROMEMO, Danish National Research Foundation, Aarhus, Denmark.

Classifications MeSH