Changes in Expression and Cellular Localization of Rat Skeletal Muscle ClC-1 Chloride Channel in Relation to Age, Myofiber Phenotype and PKC Modulation.
ClC-1
chloride ion channel 1
muscle aging
muscle development
protein kinase C (PCK)
subcellular localization
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2020
2020
Historique:
received:
28
11
2019
accepted:
30
04
2020
entrez:
6
6
2020
pubmed:
6
6
2020
medline:
6
6
2020
Statut:
epublish
Résumé
The ClC-1 chloride channel 1 is important for muscle function as it stabilizes resting membrane potential and helps to repolarize the membrane after action potentials. We investigated the contribution of ClC-1 to adaptation of skeletal muscles to needs induced by the different stages of life. We analyzed the ClC-1 gene and protein expression as well as mRNA levels of protein kinase C (PKC) alpha and theta involved in ClC-1 modulation, in soleus (SOL) and extensor digitorum longus (EDL) muscles of rats in all stage of life. The cellular localization of ClC-1 in relation to age was also investigated. Our data show that during muscle development ClC-1 expression differs according to phenotype. In fast-twitch EDL muscles ClC-1 expression increased 10-fold starting at 7 days up to 8 months of life. Conversely, in slow-twitch SOL muscles ClC-1 expression remained constant until 33 days of life and subsequently increased fivefold to reach the adult value. Aging induced a downregulation of gene and protein ClC-1 expression in both muscle types analyzed. The mRNA of PKC-theta revealed the same trend as ClC-1 except in old age, whereas the mRNA of PKC-alpha increased only after 2 months of age. Also, we found that the ClC-1 is localized in both membrane and cytoplasm, in fibers of 12-day-old rats, becoming perfectly localized on the membrane in 2-month-old rats. This study could represent a point of comparison helpful for the identification of accurate pharmacological strategies for all the pathological situations in which ClC-1 protein is altered.
Identifiants
pubmed: 32499703
doi: 10.3389/fphar.2020.00714
pmc: PMC7243361
doi:
Types de publication
Journal Article
Langues
eng
Pagination
714Informations de copyright
Copyright © 2020 Conte, Fonzino, Cibelli, De Benedictis, Imbrici, Nicchia, Pierno and Camerino.
Références
Skelet Muscle. 2015 Jul 15;5:22
pubmed: 26180627
Clin Exp Pharmacol Physiol. 2006 Nov;33(11):1118-23
pubmed: 17042925
Br J Pharmacol. 1998 Oct;125(3):477-82
pubmed: 9806330
J Gen Physiol. 2011 Mar;137(3):327-9; author reply 331-3
pubmed: 21357735
Sci Rep. 2013;3:1365
pubmed: 23455567
Neuromuscul Disord. 1993 Jul;3(4):267-74
pubmed: 8268723
Elife. 2018 May 29;7:
pubmed: 29809153
Biophys J. 2011 Sep 21;101(6):1354-63
pubmed: 21943416
J Biomed Biotechnol. 2011;2011:685328
pubmed: 22187529
Nature. 1991 Nov 28;354(6351):301-4
pubmed: 1659664
Physiology (Bethesda). 2017 Nov;32(6):425-434
pubmed: 29021362
Int J Prev Med. 2013 Jun;4(6):624-30
pubmed: 23930179
Pflugers Arch. 1994 May;427(1-2):80-5
pubmed: 8058478
Clin Chem. 2009 Apr;55(4):611-22
pubmed: 19246619
Am J Physiol Cell Physiol. 2014 Oct 1;307(7):C634-47
pubmed: 25080489
Toxicol Appl Pharmacol. 2016 Sep 1;306:36-46
pubmed: 27377005
Am J Physiol Cell Physiol. 2007 Apr;292(4):C1245-7
pubmed: 17215330
Int J Mol Sci. 2018 Nov 28;19(12):
pubmed: 30487393
Biophys J. 1996 Aug;71(2):695-706
pubmed: 8842208
J Physiol. 2016 Jun 15;594(12):3391-406
pubmed: 26857341
J Biol Chem. 1994 Nov 4;269(44):27635-9
pubmed: 7961681
PLoS One. 2014 Aug 01;9(8):e103686
pubmed: 25084151
J Pharmacol Exp Ther. 1998 Aug;286(2):903-12
pubmed: 9694949
J Biol Chem. 2009 May 15;284(20):13948-57
pubmed: 19299515
Biophys J. 2006 Dec 15;91(12):4611-22
pubmed: 17012312
Brain. 2002 Jul;125(Pt 7):1510-21
pubmed: 12077001
J Gen Physiol. 2011 Jan;137(1):21-41
pubmed: 21149546
J Pharmacol Exp Ther. 1998 Sep;286(3):1183-90
pubmed: 9732377
Exp Neurol. 2013 Oct;248:530-40
pubmed: 23933576
Exp Cell Res. 2015 Nov 15;339(1):10-9
pubmed: 26431586
Exp Neurol. 2005 Jan;191(1):163-73
pubmed: 15589523
Physiol Rev. 2018 Jul 1;98(3):1493-1590
pubmed: 29845874
PLoS One. 2013;8(2):e55930
pubmed: 23424641
Front Neurol. 2020 Feb 11;11:76
pubmed: 32117034
Front Cell Neurosci. 2015 Apr 27;9:156
pubmed: 25964741
Pflugers Arch. 1989 Mar;413(5):568-70
pubmed: 2740209
Pharmacol Res. 2010 Jun;61(6):553-63
pubmed: 20116431
Diagn Pathol. 2017 Feb 20;12(1):19
pubmed: 28219397
Front Pharmacol. 2017 Mar 23;8:151
pubmed: 28386229
Dev Biol. 1993 Jun;157(2):359-70
pubmed: 8500649
FEBS Lett. 1999 Apr 16;449(1):12-6
pubmed: 10225418
Pflugers Arch. 1990 Apr;416(1-2):17-21
pubmed: 2162030
FASEB J. 2004 May;18(7):905-7
pubmed: 15033928
Biol Cell. 2003 Sep;95(6):399-406
pubmed: 14519557
J Neurosci. 2004 Apr 21;24(16):4070-81
pubmed: 15102922
J Gen Physiol. 2009 Oct;134(4):309-22
pubmed: 19786584
J Gen Physiol. 2011 Jan;137(1):17-9
pubmed: 21149545
Front Physiol. 2014 Oct 07;5:378
pubmed: 25339907
Sci Rep. 2019 Feb 28;9(1):3185
pubmed: 30816241
Sci Rep. 2016 Sep 01;6:32444
pubmed: 27580824
Antioxid Redox Signal. 2018 Apr 20;28(12):1105-1119
pubmed: 28931313
Cell Physiol Biochem. 2010;25(6):687-94
pubmed: 20511714
Am J Physiol Cell Physiol. 2011 Apr;300(4):C723-42
pubmed: 21209361
Age (Dordr). 2014 Feb;36(1):73-88
pubmed: 23716142
J Gen Physiol. 2016 Apr;147(4):291-308
pubmed: 27022190
J Physiol. 1999 Feb 1;514 ( Pt 3):677-85
pubmed: 9882739
Exp Eye Res. 2016 May;146:259-68
pubmed: 27018215
Br J Pharmacol. 2018 May;175(10):1770-1780
pubmed: 29500929
F1000Res. 2018 Dec 11;7:
pubmed: 30631434
Pflugers Arch. 2014 Dec;466(12):2215-28
pubmed: 24643479
Sci Rep. 2015 May 29;5:10667
pubmed: 26021757
Brain. 2017 Sep 1;140(9):2295-2305
pubmed: 29050397
Glia. 2004 Jul;47(1):46-57
pubmed: 15139012
J Gen Physiol. 2010 Dec;136(6):597-613
pubmed: 21078869
JAMA. 2018 Dec 11;320(22):2344-2353
pubmed: 30535218
Neurotherapeutics. 2018 Oct;15(4):954-965
pubmed: 30341599
PLoS Biol. 2019 Apr 25;17(4):e3000218
pubmed: 31022181
Neurobiol Dis. 2005 Mar;18(2):356-65
pubmed: 15686964
Skelet Muscle. 2011 Jul 13;1(1):26
pubmed: 21797990
Am J Physiol Cell Physiol. 2007 Apr;292(4):C1291-7
pubmed: 17135300
PLoS One. 2015 Jun 11;10(6):e0129686
pubmed: 26066046
J Microsc. 2013 Dec;252(3):295-302
pubmed: 24117417
Mol Biol Cell. 2006 Jul;17(7):3156-75
pubmed: 16641372
J Physiol. 2007 Nov 1;584(Pt 3):983-95
pubmed: 17855757
J Appl Physiol (1985). 2018 Aug 1;125(2):470-478
pubmed: 29722626