Neutral DNA-avidin nanoparticles as ultrasensitive reporters in immuno-PCR.
Journal
The Analyst
ISSN: 1364-5528
Titre abrégé: Analyst
Pays: England
ID NLM: 0372652
Informations de publication
Date de publication:
21 Jul 2020
21 Jul 2020
Historique:
pubmed:
6
6
2020
medline:
15
5
2021
entrez:
6
6
2020
Statut:
ppublish
Résumé
We have developed an immuno-PCR based diagnostic platform which couples detection antibodies to self-assembled, ultra-detectable DNA-avidin nanoparticles stabilized with poly(ethylene glycol) to link DNA amplification to target protein concentration. Electrostatic neutralization and cloaking of the PCR-amplifiable DNA labels by avidin and PEG coating reduces non-specific "stickiness" and enhances assay sensitivity. We further optimized the detectability of the nanoparticles by incorporating four repeats of a unique synthetic DNA PCR target into each nanoparticle. Using human chorionic gonadotropin hormone (hCG) as a model analyte, this platform was able to quantitate the target hCG protein in femtomolar concentrations using only standard laboratory equipment.
Identifiants
pubmed: 32500871
doi: 10.1039/d0an00134a
pmc: PMC7412439
mid: NIHMS1601883
doi:
Substances chimiques
Antibodies
0
Avidin
1405-69-2
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4942-4949Subventions
Organisme : NIAID NIH HHS
ID : R21 AI111120
Pays : United States
Références
J Phys Chem B. 2012 Jan 12;116(1):621-32
pubmed: 22091866
J Med Microbiol. 2014 May;63(Pt 5):627-641
pubmed: 24568881
J Phys Chem B. 2015 Aug 27;119(34):11030-40
pubmed: 25966319
Biomol Detect Quantif. 2015 May 20;4:10-6
pubmed: 27077033
PLoS One. 2012;7(12):e51863
pubmed: 23284792
Bioanalysis. 2017 May;9(9):753-764
pubmed: 28488883
Anal Chim Acta. 2016 Mar 3;910:12-24
pubmed: 26873464
Biophys J. 2012 Apr 18;102(8):1881-8
pubmed: 22768944
Mol Immunol. 2018 Jun;98:28-35
pubmed: 29325980
Biophys J. 2002 Jan;82(1 Pt 1):357-65
pubmed: 11751322
Science. 1992 Oct 2;258(5079):120-2
pubmed: 1439758
J Cell Biol. 1982 May;93(2):298-305
pubmed: 7096440
Anal Chem. 2012 Apr 3;84(7):3433-9
pubmed: 22414051
J Clin Invest. 1959 Nov;38:1996-2016
pubmed: 13799922
Methods Enzymol. 1990;184:51-67
pubmed: 2388586
Environ Health Perspect. 1987 Oct;74:57-66
pubmed: 3319556
J Mol Recognit. 2004 Nov-Dec;17(6):558-66
pubmed: 15386619
Nature. 1976 Jan 29;259(5541):333-5
pubmed: 1250371
Nucleic Acids Res. 1995 Feb 11;23(3):522-9
pubmed: 7885849
Analyst. 2016 Dec 19;142(1):91-97
pubmed: 27731431
Clin Chem. 2008 Nov;54(11):1840-3
pubmed: 18787014
Anal Chem. 2014 Aug 5;86(15):7471-7
pubmed: 24992514
Sci Rep. 2015 Feb 13;5:8442
pubmed: 25676333
Lab Chip. 2018 Jan 30;18(3):522-531
pubmed: 29326990
J Pharmacol Toxicol Methods. 2011 May-Jun;63(3):227-35
pubmed: 21147239
Biochemistry. 1999 Oct 19;38(42):14069-76
pubmed: 10529254
Biotechnol Lett. 2014 Sep;36(9):1863-8
pubmed: 24930095
Science. 1993 Apr 30;260(5108):699
pubmed: 17812229
Anal Biochem. 2019 Apr 1;570:56-61
pubmed: 30768924
J Immunol Res. 2016;2016:6262383
pubmed: 27556048
ACS Appl Mater Interfaces. 2018 Sep 26;10(38):31845-31849
pubmed: 30168312
Bioanalysis. 2018 Jun 1;10(12):909-912
pubmed: 29923752
J Nanobiotechnology. 2012 Jun 22;10(1):26
pubmed: 22726242
Trends Microbiol. 2011 Jun;19(6):295-302
pubmed: 21478019
Analyst. 2008 Jun;133(6):702-18
pubmed: 18493669
Vet Q. 2016 Jun;36(2):63-70
pubmed: 27018241
Nat Biotechnol. 2010 Jun;28(6):595-9
pubmed: 20495550
J Chromatogr A. 2003 Jan 17;984(2):215-21
pubmed: 12564692
Chem Commun (Camb). 2013 May 8;49(36):3778-80
pubmed: 23539517
PLoS One. 2016 Mar 23;11(3):e0152002
pubmed: 27008630
Bioconjug Chem. 2010 Jul 21;21(7):1254-63
pubmed: 20527874