Strategies for Chalcogenide Thin Film Functionalization.

We report the functionalization of chalcogenide thin films with biotinylated 12-mer peptides SVSVGMKPSPRP and LLADTTHHRPWT exhibiting a high binding affinity toward inorganic surfaces, on the one hand, and with (3-aminopropyl)triethoxysilane (APTES), on the other hand. The specific biotin moieties were used to bind streptavidin proteins and demonstrate the efficacy of the biofunctionalizated chalcogenide thin films to capture biomolecules. Atomic force microscopy provided high-resolution images of the interfaces, and water contact angle measurements gave insight into the interaction mechanisms. Fourier transform infrared spectroscopy in attenuated total reflection mode provided information about the secondary structure of the bound proteins, thanks to the deconvolution of the amide I band (1700-1600 cm