Comparative evaluation of different antigen detection methods for the detection of peste des petits ruminants virus.
Small ruminant morbillivirus
diagnostic
goats
pathogenesis
peste des petits ruminants (PPR)
rapid detection methods
virus
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
22
12
2019
revised:
15
05
2020
accepted:
28
05
2020
pubmed:
6
6
2020
medline:
9
1
2021
entrez:
6
6
2020
Statut:
ppublish
Résumé
Peste des petits ruminants (PPR) is a fatal disease of small ruminants which has spread rapidly to previously PPR-free countries in recent decades, causing enormous economic losses in the affected regions. Here, two newly emerged PPR virus (PPRV) isolates from India and from the Middle East were tested in an animal trial to analyse their pathogenesis, and to evaluate serological and molecular detection methods. Animals infected with the two different PPRV isolates showed marked differences in clinical manifestation and scoring. The PPRV isolate from India was less virulent than the virus from the Middle East. Commercially available rapid detection methods for PPRV antigen (two Lateral Flow Devices (LFDs) and one antigen ELISA) were evaluated in comparison with a nucleic acid detection method. For this purpose, ocular and nasal swabs were used. Due to the easy non-invasive sampling, faecal samples were also analysed. For all rapid antigen detection methods, a high specificity of 100% was observed independent of the sample matrix and dilution buffers used. Both antigen ELISA and LFD tests showed highest sensitivities for nasal swabs. Here, the detection rate of the antigen ELISA, the LFD-PESTE-TEST and the LFD-ID Rapid-Test was 78%, 75% and 78%, respectively. Ocular swabs were less suitable for antigen detection of PPRV. These results reflect the increased viral load in nasal swabs of PPRV infected goats compared to ocular swabs. The faecal samples were the least suitable for antigen detection. In conclusion, nasal swab samples are the first choice for the antigen and genome detection of PPRV. Nevertheless, based on the excellent diagnostic specificity of the rapid tests, positive results generated with other sample matrices are solid. In contrast, negative test results can be caused on the reduced analytical sensitivity of the rapid antigen tests and must be treated with caution.
Substances chimiques
Antigens, Viral
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2881-2891Informations de copyright
© 2020 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.
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