Role of metal speciation in the exposure medium on the toxicity, bioavailability and bio-reactivity of copper, silver, cadmium and zinc in the rainbow trout gut cell line (RTgutGC).
Animals
Biological Availability
Cadmium
/ pharmacokinetics
Cation Transport Proteins
/ genetics
Cells, Cultured
Copper
/ pharmacokinetics
Copper-Transporting ATPases
/ genetics
Gastrointestinal Microbiome
/ drug effects
Metallothionein
/ genetics
Oncorhynchus mykiss
/ physiology
Silver
/ metabolism
Zinc
/ pharmacokinetics
In vitro alternative
Metal bioavailability
Metal bioreactivity
Metal speciation
Metal toxicity
Journal
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
ISSN: 1532-0456
Titre abrégé: Comp Biochem Physiol C Toxicol Pharmacol
Pays: United States
ID NLM: 100959500
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
14
02
2020
revised:
24
05
2020
accepted:
29
05
2020
pubmed:
6
6
2020
medline:
20
2
2021
entrez:
6
6
2020
Statut:
ppublish
Résumé
The role of metal speciation on metal bioavailability, bio-reactivity and toxicity at the fish intestine is poorly understood. To investigate these processes, we used an in vitro model of the rainbow trout (Oncorhynchus mykiss) intestine, the RTgutGC cell line. Cells were exposed to two essential metals (copper and zinc) and two non-essential metals (cadmium and silver) in a medium of well-defined composition, which allowed the determination of metal speciation in solution. Concentrations resulting in a 50% cell viability reduction (EC
Identifiants
pubmed: 32502601
pii: S1532-0456(20)30116-2
doi: 10.1016/j.cbpc.2020.108816
pii:
doi:
Substances chimiques
Cation Transport Proteins
0
Cadmium
00BH33GNGH
Silver
3M4G523W1G
Copper
789U1901C5
Metallothionein
9038-94-2
Copper-Transporting ATPases
EC 7.2.2.8
Zinc
J41CSQ7QDS
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108816Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.