Eupatilin Promotes Cell Death by Calcium Influx through ER-Mitochondria Axis with SERPINB11 Inhibition in Epithelial Ovarian Cancer.
ER–mitochondria axis
SERPINB11
calcium influx
eupatilin
ovarian cancer
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
03 Jun 2020
03 Jun 2020
Historique:
received:
27
04
2020
revised:
26
05
2020
accepted:
31
05
2020
entrez:
7
6
2020
pubmed:
7
6
2020
medline:
7
6
2020
Statut:
epublish
Résumé
Ovarian cancer is the leading cause of gynecological cancer-related mortality. The anticancer effect of eupatilin, a family of flavonoids, is known in many cancer types, but it is unclear what mechanism it plays in ovarian cancer. In this study, eupatilin promoted cell death of ovarian cancer cells by activating caspases, cell cycle arrest, reactive oxygen species (ROS) generation, calcium influx, disruption of the endoplasmic reticulum (ER)-mitochondria axis with SERPINB11 inhibition, and downregulation of phosphoinositide 3-kinase (PI3K) and mitogen activated protein kinase (MAPK) pathways. Additionally, eupatilin-reduced SERPINB11 expression enhanced the effect of conventional chemotherapeutic agents against ovarian cancer cell progression. Cotreatment with siSERPINB11 and eupatilin increased calcium-ion-dependent apoptotic activity in ovarian cancer cells. Although there were no significant toxic effects of eupatilin on embryos, eupatilin completely inhibited tumorigenesis in a zebrafish xenograft model. In addition, eupatilin suppressed angiogenesis in zebrafish transgenic models. Collectively, downregulating SERPINB11 with eupatilin against cancer progression may improve therapeutic activity.
Identifiants
pubmed: 32503295
pii: cancers12061459
doi: 10.3390/cancers12061459
pmc: PMC7353024
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Environ Pathol Toxicol Oncol. 2005;24(4):261-9
pubmed: 16393120
Int Immunopharmacol. 2019 Oct;75:105748
pubmed: 31306979
Biomed Pharmacother. 2018 Jul;103:135-139
pubmed: 29649628
Biochemistry. 1998 Apr 14;37(15):5258-66
pubmed: 9548757
J Biol Chem. 2013 Jan 18;288(3):1612-9
pubmed: 23195956
CA Cancer J Clin. 2015 Jan-Feb;65(1):5-29
pubmed: 25559415
Front Oncol. 2017 Aug 31;7:180
pubmed: 28913175
Am J Pathol. 2011 Sep;179(3):1110-9
pubmed: 21723846
Hematol Oncol Clin North Am. 2003 Aug;17(4):909-25, vii
pubmed: 12959182
Korean J Physiol Pharmacol. 2014 Oct;18(5):383-90
pubmed: 25352757
Eur J Cancer. 2013 Apr;49(6):1374-403
pubmed: 23485231
Genome Biol. 2006;7(5):216
pubmed: 16737556
Biomed Res Int. 2019 Apr 24;2019:5016483
pubmed: 31179326
Autophagy. 2017 Jul 3;13(7):1250-1251
pubmed: 28548902
Hum Genomics. 2013 Oct 30;7:22
pubmed: 24172014
Int J Cancer. 2014 Apr 1;134(7):1595-604
pubmed: 24114705
Differentiation. 2009 Apr;77(4):412-23
pubmed: 19281788
Oncol Lett. 2016 Oct;12(4):2894-2899
pubmed: 27698876
Oncol Rep. 2018 Jun;39(6):2942-2950
pubmed: 29693162
Science. 1994 Jan 28;263(5146):526-9
pubmed: 8290962
J Gastric Cancer. 2011 Mar;11(1):16-22
pubmed: 22076197
J Biol Chem. 2001 Sep 7;276(36):33293-6
pubmed: 11435447
Front Oncol. 2017 Jul 05;7:140
pubmed: 28725634
Biomed Pharmacother. 2019 Aug;116:109004
pubmed: 31128404
Gynecol Oncol. 2014 Apr;133(1):128-37
pubmed: 24434059
Oncogene. 2018 Jan 18;37(3):403-414
pubmed: 28967908
Bioorg Med Chem Lett. 2018 Oct 15;28(19):3150-3154
pubmed: 30177376
J Ethnopharmacol. 2008 Jun 19;118(1):94-101
pubmed: 18440740
Cell Tissue Res. 2014 Jul;357(1):373-80
pubmed: 24781146
Exp Biol Med (Maywood). 2012 Feb;237(2):150-9
pubmed: 22289513
Food Chem Toxicol. 2011 Aug;49(8):1737-44
pubmed: 21554918
Mol Med Rep. 2016 Feb;13(2):1141-6
pubmed: 26676446
Cell Biochem Funct. 2020 Jun;38(4):428-435
pubmed: 31926121
Cancer Manag Res. 2019 May 24;11:4781-4796
pubmed: 31213900
Int J Oncol. 2016 Sep;49(3):1148-54
pubmed: 27573489
Oncol Rep. 2010 Jul;24(1):241-9
pubmed: 20514468
Tumour Biol. 2013 Apr;34(2):875-85
pubmed: 23292941
Evid Based Complement Alternat Med. 2015;2015:483980
pubmed: 26539226
BMC Nephrol. 2018 Jun 15;19(1):140
pubmed: 29907098
Biochim Biophys Acta. 1999 Jan 12;1416(1-2):92-100
pubmed: 9889332
Redox Biol. 2015 Dec;6:260-271
pubmed: 26296072
J Cell Biochem. 2009 Sep 1;108(1):225-36
pubmed: 19565564
Gynecol Oncol. 2015 Apr;137(1):173-9
pubmed: 25677064