Biomarker considerations in monitoring petrogenic pollution using the mussel Mytilus galloprovincialis.
Antioxidant enzymes
Biomarkers
CYPs
Carboxylesterases
Mussels
PAHs
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
05
02
2020
accepted:
22
05
2020
pubmed:
7
6
2020
medline:
8
8
2020
entrez:
7
6
2020
Statut:
ppublish
Résumé
Mussels are worldwide bioindicators in pollution monitoring since they fulfil the requirements for being good sentinels. However, some methodological concerns arise in the use of particular biomarkers, particularly those displaying low enzymatic rates and/or limited responsiveness to chemicals and biological-related variability. In the present study, the suitability of oxidative stress and detoxification parameters when using mussels as sentinels of polycyclic aromatic hydrocarbon (PAH) pollution is addressed. Present results show that the S9 subcellular fraction of the digestive gland in mussels is an adequate and convenient matrix where to measure most pollution-related biomarkers. Furthermore, this work constitutes the first evidence of the potential suitability of using particular carboxylesterase (CE) activities in determining PAHs exposure in mussels. This fact could imply the replacement of more controversial cytochrome P450 components (phase I oxidation), which are only measurable in microsomal fractions, by CEs (measured in S9 fractions) as good alternatives for phase I reactions in PAH-exposed mussels. Some methodological considerations, such as the need of including commercial purified proteins in biomarker determinations for quality assurance, are evaluated.
Identifiants
pubmed: 32504435
doi: 10.1007/s11356-020-09427-3
pii: 10.1007/s11356-020-09427-3
doi:
Substances chimiques
Biomarkers
0
Polycyclic Aromatic Hydrocarbons
0
Water Pollutants, Chemical
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
31854-31862Subventions
Organisme : Ministerio de Economía y Competitividad
ID : CGL2016-76332-R
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