Uncovering an Organ's Molecular Architecture at Single-Cell Resolution by Spatially Resolved Transcriptomics.
fluorescence in situ hybridization
in situ sequencing
single-cell RNA-seq
spatial reconstruction
spatial transcriptomics
spatially resolved transcriptomics
Journal
Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
28
12
2019
revised:
11
05
2020
accepted:
12
05
2020
pubmed:
9
6
2020
medline:
23
9
2021
entrez:
8
6
2020
Statut:
ppublish
Résumé
Revealing fine-scale cellular heterogeneity among spatial context and the functional and structural foundations of tissue architecture is fundamental within biological research and pharmacology. Unlike traditional approaches involving single molecules or bulk omics, cutting-edge, spatially resolved transcriptomics techniques offer near-single-cell or even subcellular resolution within tissues. Massive information across higher dimensions along with position-coordinating labels can better map the whole 3D transcriptional landscape of tissues. In this review, we focus on developments and strategies in spatially resolved transcriptomics, compare the cell and gene throughput and spatial resolution in detail for existing methods, and highlight the enormous potential in biomedical research.
Identifiants
pubmed: 32505359
pii: S0167-7799(20)30140-2
doi: 10.1016/j.tibtech.2020.05.006
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
43-58Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.