Monitoring global chromatin dynamics in response to DNA damage.
Chromatin motion
Correlated motion
DNA damage
Double-strand break
Microscopy
Journal
Mutation research
ISSN: 1873-135X
Titre abrégé: Mutat Res
Pays: Netherlands
ID NLM: 0400763
Informations de publication
Date de publication:
Historique:
received:
03
11
2019
revised:
08
05
2020
accepted:
16
05
2020
pubmed:
9
6
2020
medline:
15
12
2020
entrez:
8
6
2020
Statut:
ppublish
Résumé
DNA damage induced global chromatin motion has been observed in yeast and mammalian cells. Currently, it is unclear what mechanisms may be driving these changes in whole genome dynamics. Recent advances in live-cell microscopy now enable chromatin motion to be quantified throughout the whole nucleus. In addition, much work has improved quantification of single particle trajectories. This topic is particularly important to the field of DNA repair as there are a large number of unanswered questions that can be tackled by monitoring global chromatin movement. Foremost, is how local DNA repair mechanisms interact and change global chromatin structure and whether this impacts repair pathway choice or efficiency. In this review, we describe methodologies to monitor global chromatin movement putting them into context with the DNA repair field highlighting how these techniques can drive new discoveries.
Identifiants
pubmed: 32505939
pii: S0027-5107(20)30040-3
doi: 10.1016/j.mrfmmm.2020.111707
pii:
doi:
Substances chimiques
Chromatin
0
Histones
0
Nucleosomes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
111707Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.