Characteristics of bull sperm acrosome associated 1 proteins.
Acrosome
Cattle
Epididymis
Sperm
Subfertility
Journal
Animal reproduction science
ISSN: 1873-2232
Titre abrégé: Anim Reprod Sci
Pays: Netherlands
ID NLM: 7807205
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
23
10
2019
revised:
23
04
2020
accepted:
23
04
2020
entrez:
9
6
2020
pubmed:
9
6
2020
medline:
7
4
2021
Statut:
ppublish
Résumé
An atypical distribution of sperm acrosomal tyrosine-phosphorylated proteins [which include sperm acrosome associated 1 (SPACA1) proteins] may be related to the relatively lesser pregnancy rates when semen of some bulls are used for artificial insemination (AI). There may also be these associations with bull SPACA1 proteins that are translocated from the equatorial segment to the anterior part in the acrosomes during sperm maturation in the normally functioning epididymis. The aim of the present study, therefore, was assessment of the characteristics of bull SPACA1 proteins. Results from immunocytochemical evaluations indicate there were large variations in sperm percentages with typically distributed SPACA1 proteins in acrosomes of cauda epididymal sperm samples (7%-95%). These values were positively correlated with percentages of epididymal spermatozoa with typically distributed acrosomal tyrosine-phosphorylated proteins (r=0.8564, P<0.001). Results indicate there are individual differences in translocation of SPACA1 proteins in the epididymis during sperm maturation, and that SPACA1 protein is one of the main determinants for the typical distribution of acrosomal tyrosine-phosphorylated proteins. In addition, conception rates as a result of AI using cryopreserved spermatozoa tended to be associated with percentages of epididymal spermatozoa with typically distributed SPACA1 proteins. Results from sucrose gradient centrifugation fractionation experiments indicate SPACA1 proteins are sperm membrane raft-associated proteins. Based on these results, it is hypothesized that there is an association between bull subfertility when semen is used for AI and epididymal dysfunctions in the arrangement of membrane lipid rafts during sperm maturation.
Identifiants
pubmed: 32507260
pii: S0378-4320(20)30351-1
doi: 10.1016/j.anireprosci.2020.106479
pii:
doi:
Substances chimiques
Isoantigens
0
Seminal Plasma Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106479Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest This research was supported in part by the Grants-in-Aid from Japan Society for the Promotion of Science to H.H. (grant number 16K07990, 19K22366).