Physiologically Relevant Fluid-Induced Oscillatory Shear Stress Stimulation of Mesenchymal Stem Cells Enhances the Engineered Valve Matrix Phenotype.
endothelial phenotype
engineered valve
mechanical conditioning
mesenchymal stem cells
oscillatory flow
physiologically relevant
smooth muscle phenotype
somatic growth
Journal
Frontiers in cardiovascular medicine
ISSN: 2297-055X
Titre abrégé: Front Cardiovasc Med
Pays: Switzerland
ID NLM: 101653388
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
12
2019
accepted:
07
04
2020
entrez:
9
6
2020
pubmed:
9
6
2020
medline:
9
6
2020
Statut:
epublish
Résumé
Support of somatic growth is a fundamental requirement of tissue-engineered valves. However, efforts thus far have been unable to maintain this support long term. A key event that will determine the valve's long-term success is the extent to which healthy host tissue remodeling can occur on the valve soon after implantation. The construct's phenotypic-status plays a critical role in accelerating tissue remodeling and engineered valve integration with the host via chemotaxis. In the current study, human bone-marrow-derived mesenchymal stem cells were utilized to seed synthetic, biodegradable scaffolds for a period of 8 days in rotisserie culture. Subsequently, cell-seeded scaffolds were exposed to physiologically relevant oscillatory shear stresses (overall mean, time-averaged shear stress, ~7.9 dynes/cm
Identifiants
pubmed: 32509802
doi: 10.3389/fcvm.2020.00069
pmc: PMC7248568
doi:
Types de publication
Journal Article
Langues
eng
Pagination
69Informations de copyright
Copyright © 2020 Gonzalez, Perez-Nevarez, Mirza, Perez, Lin, Hsu, Caobi, Raymond, Gomez Hernandez, Fernandez-Lima, George and Ramaswamy.
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