Rapid detection and quantification of plasmid-mediated colistin resistance genes (mcr-1 to mcr-5) by real-time PCR in bacterial and environmental samples.
E. coli (all potentially pathogenic types)
antimicrobials
detection
environmental
resistance
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
12
11
2019
revised:
18
05
2020
accepted:
02
06
2020
pubmed:
9
6
2020
medline:
19
12
2020
entrez:
9
6
2020
Statut:
ppublish
Résumé
The aim of the study was to validate a rapid method to detect and quantify colistin resistance genes (mcr-1 to mcr-5) by real-time polymerase chain reaction (RT-PCR) in diverse matrices. The detection limit of two newly designed SYBR Green real-time PCR assays for mcr-4 and mcr-5 and of previously published protocols for mcr-1 to mcr-3 was assessed using serial dilutions of reference strains. The assays could detect all five mcr genes with the lower limit of 10 This method represents a set of sensitive, rapid and effective assays for the screening of colistin resistance directly from the environment. Colistin is an antimicrobial commonly used in animals and has recently emerged as a last-resort treatment in humans. Plasmid-mediated mcr genes confer resistance to colistin and represent a major threat for public health since they can be easily disseminated through horizontal gene transfer. The rapid and sensitive detection of mcr genes is of utmost necessity.
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Colistin
Z67X93HJG1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1523-1529Subventions
Organisme : Italian Ministry of Research and University
ID : 2015KA3XFH
Informations de copyright
© 2020 The Society for Applied Microbiology.
Références
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