Prioritization of SARS-CoV-2 epitopes using a pan-HLA and global population inference approach.

SARS-CoV-2 T cells epitope prediction public resource

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
29 Jun 2020
Historique:
pubmed: 9 6 2020
medline: 9 6 2020
entrez: 9 6 2020
Statut: epublish

Résumé

SARS-CoV-2 T cell response assessment and vaccine development may benefit from an approach that considers the global landscape of the human leukocyte antigen (HLA) proteins. We predicted the binding affinity between 9-mer and 15-mer peptides from the SARS-CoV-2 peptidome for 9,360 class I and 8,445 class II HLA alleles, respectively. We identified 368,145 unique combinations of peptide-HLA complexes (pMHCs) with a predicted binding affinity less than 500nM, and observed significant overlap between class I and II predicted pMHCs. Using simulated populations derived from worldwide HLA frequency data, we identified sets of epitopes predicted in at least 90% of the population in 57 countries. We also developed a method to prioritize pMHCs for specific populations. Collectively, this public dataset and accessible user interface (Shiny app: https://rstudio-connect.parkerici.org/content/13/) can be used to explore the SARS-CoV-2 epitope landscape in the context of diverse HLA types across global populations.

Identifiants

pubmed: 32511325
doi: 10.1101/2020.03.30.016931
pmc: PMC7239055
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NCATS NIH HHS
ID : KL2 TR001882
Pays : United States

Déclaration de conflit d'intérêts

Declaration of Interests K.M.C is a shareholder in Geneoscopy LLC. D.K.W. is a founder, equity holder and receives consulting fees from Immunai. A.R. is supported by the National Institute of Health (R35 CA197633), the Ressler Family Fund, the Agilent Thought Leader Award, a Stand Up to Cancer- Bristol-Meyer Squibb Catalyst Research Grant (Grant Number: SU2C-AACR-CT06–17). This research grant is administered by the American Association for Cancer Research, the scientific partner of SU2C. A.R. is a member researcher at the Parker Institute for Cancer Immunotherapy.

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Auteurs

Katie M Campbell (KM)

Department of Medicine, Division of Hematology-Oncology, University of California, Los Angeles (UCLA), Los Angeles, CA, 90095, USA.
These authors contributed equally to this work.
Senior author.
Lead Contact.

Gabriela Steiner (G)

Parker Institute for Cancer Immunotherapy, San Francisco, CA, 94129, USA.
These authors contributed equally to this work.

Daniel K Wells (DK)

Parker Institute for Cancer Immunotherapy, San Francisco, CA, 94129, USA.

Antoni Ribas (A)

Department of Medicine, Division of Hematology-Oncology, University of California, Los Angeles (UCLA), Los Angeles, CA, 90095, USA.
Parker Institute for Cancer Immunotherapy, San Francisco, CA, 94129, USA.
Department Surgery, Division of Surgical Oncology, University of California, Los Angeles, Los Angeles, CA, USA.
Jonsson Comprehensive Cancer Center, Los Angeles, CA, USA.

Anusha Kalbasi (A)

Department Surgery, Division of Surgical Oncology, University of California, Los Angeles, Los Angeles, CA, USA.
Jonsson Comprehensive Cancer Center, Los Angeles, CA, USA.
Department of Radiation Oncology, UCLA, CA, 90095, USA.
Senior author.

Classifications MeSH