Human immunoglobulin G responses to Cimex lectularius L. saliva.
Cimex lectularius
IgG
antibody
bed bug
cimicosis
immunoglobulin
saliva
Journal
Parasite immunology
ISSN: 1365-3024
Titre abrégé: Parasite Immunol
Pays: England
ID NLM: 7910948
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
29
04
2020
revised:
02
06
2020
accepted:
03
06
2020
pubmed:
10
6
2020
medline:
13
2
2021
entrez:
10
6
2020
Statut:
ppublish
Résumé
To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract. Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.
Identifiants
pubmed: 32516446
doi: 10.1111/pim.12764
pmc: PMC7668222
mid: NIHMS1615533
doi:
Substances chimiques
Immunoglobulin G
0
Salivary Proteins and Peptides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12764Subventions
Organisme : NIH HHS
ID : P51 OD011104
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR000439
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002548
Pays : United States
Organisme : NCATS NIH HHS
ID : TR000439
Pays : United States
Informations de copyright
© 2020 John Wiley & Sons Ltd.
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