Infectious Norovirus Is Chronically Shed by Immunocompromised Pediatric Hosts.
Adolescent
Adult
Caliciviridae Infections
/ immunology
Carrier State
/ transmission
Child
Child, Preschool
Cross Infection
/ transmission
Feces
/ virology
Female
Gastroenteritis
/ virology
Humans
Immunocompromised Host
Infant
Male
Norovirus
/ genetics
Pediatrics
/ statistics & numerical data
Prospective Studies
Seasons
Virus Shedding
Young Adult
asymptomatic
diarrhea
genotype
immunocompromised host
infectious virus shedding
norovirus
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
05 06 2020
05 06 2020
Historique:
received:
20
04
2020
revised:
28
05
2020
accepted:
02
06
2020
entrez:
11
6
2020
pubmed:
11
6
2020
medline:
13
2
2021
Statut:
epublish
Résumé
Noroviruses are a leading cause of gastroenteritis worldwide. Although infections in healthy individuals are self-resolving, immunocompromised individuals are at risk for chronic disease and severe complications. Chronic norovirus infections in immunocompromised hosts are often characterized by long-term virus shedding, but it is unclear whether this shed virus remains infectious. We investigated the prevalence, genetic heterogeneity, and temporal aspects of norovirus infections in 1140 patients treated during a 6-year period at a pediatric research hospital. Additionally, we identified 20 patients with chronic infections lasting 37 to >418 days. Using a new human norovirus in vitro assay, we confirmed the continuous shedding of infectious virus for the first time. Shedding lasted longer in male patients and those with diarrheal symptoms. Prolonged shedding of infectious norovirus in immunocompromised hosts can potentially increase the likelihood of transmission, highlighting the importance of isolation precautions to prevent nosocomial infections.
Identifiants
pubmed: 32516960
pii: v12060619
doi: 10.3390/v12060619
pmc: PMC7354526
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI141478
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI106700
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI123144
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI116482
Pays : United States
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI123144-01
Pays : International
Organisme : National Institute of Allergy and Infectious Diseases
ID : AI125254-01
Pays : International
Références
J Virol Methods. 2015 Oct;223:109-14
pubmed: 26248055
J Clin Microbiol. 2017 Jul;55(7):2208-2221
pubmed: 28490488
Epidemiol Infect. 2000 Jun;124(3):481-7
pubmed: 10982072
J Clin Virol. 2002 Aug;25(2):233-5
pubmed: 12367660
Biol Blood Marrow Transplant. 2012 Dec;18(12):1883-9
pubmed: 22796532
J Infect Dis. 2012 Oct;206(8):1269-74
pubmed: 22872736
Emerg Infect Dis. 2018 Aug;24(8):1453-1464
pubmed: 30014841
FASEB J. 2002 Jun;16(8):869-71
pubmed: 11967234
Diagn Microbiol Infect Dis. 2018 Oct;92(2):143-146
pubmed: 29934072
Appl Environ Microbiol. 2003 Oct;69(10):5782-6
pubmed: 14532025
J Virol Methods. 2002 Feb;100(1-2):107-14
pubmed: 11742657
Emerg Infect Dis. 2008 Aug;14(8):1224-31
pubmed: 18680645
Emerg Infect Dis. 2008 Oct;14(10):1553-7
pubmed: 18826818
J Infect Dis. 2008 Oct 1;198(7):994-1001
pubmed: 18774885
J Clin Virol. 2011 Jun;51(2):121-5
pubmed: 21514213
J Infect Dis. 1997 Nov;176(5):1374-8
pubmed: 9359742
J Clin Microbiol. 2010 Nov;48(11):4303-5
pubmed: 20810762
Am J Trop Med Hyg. 2001 Jul;65(1):57-63
pubmed: 11504409
J Virol Methods. 2004 Mar 15;116(2):109-17
pubmed: 14738976
Lancet. 2004 Feb 28;363(9410):682-8
pubmed: 15001325
Clin Infect Dis. 2010 Aug 1;51(3):307-14
pubmed: 20575662
Curr Opin Infect Dis. 2019 Aug;32(4):348-355
pubmed: 31107251
Open Forum Infect Dis. 2016 Sep 08;3(3):ofw169
pubmed: 27800529
J Clin Microbiol. 2005 Sep;43(9):4836-9
pubmed: 16145153
PLoS Pathog. 2019 Oct 31;15(10):e1008057
pubmed: 31671153
J Gen Virol. 2019 Oct;100(10):1393-1406
pubmed: 31483239
Emerg Infect Dis. 2005 Jul;11(7):1079-85
pubmed: 16022784
mBio. 2013 Jul 16;4(4):
pubmed: 23860770
Clin Microbiol Infect. 2014 Aug;20(8):717-23
pubmed: 25040790
Science. 2014 Nov 7;346(6210):755-9
pubmed: 25378626
J Med Virol. 2014 Jul;86(7):1203-9
pubmed: 24115094
J Pediatric Infect Dis Soc. 2013 Mar;2(1):57-60
pubmed: 23687584
PLoS One. 2013 Oct 02;8(10):e75922
pubmed: 24098406
Science. 2016 Sep 23;353(6306):1387-1393
pubmed: 27562956
Emerg Infect Dis. 2015 Apr;21(4):592-9
pubmed: 25811368
PLoS Med. 2008 Feb;5(2):e31
pubmed: 18271619
N Engl J Med. 2012 Nov 29;367(22):2126-32
pubmed: 23190223
Lancet Infect Dis. 2014 Aug;14(8):725-730
pubmed: 24981041
Viruses. 2019 Jun 08;11(6):
pubmed: 31181749
J Virol. 2015 Jun;89(11):5756-9
pubmed: 25787285
Int J Food Microbiol. 2006 May 1;108(3):391-6
pubmed: 16499983
Clin Infect Dis. 2018 Aug 1;67(4):541-548
pubmed: 29518185
J Virol. 2014 Dec;88(24):14184-96
pubmed: 25275120
J Infect Dis. 2017 Dec 19;216(12):1513-1524
pubmed: 29029115
J Med Virol. 2011 Jun;83(6):1078-85
pubmed: 21503924
J Med Virol. 2010 Apr;82(4):632-7
pubmed: 20166189
J Virol. 2017 Mar 13;91(7):
pubmed: 28100612
Nat Protoc. 2015 Dec;10(12):1939-47
pubmed: 26513671