Material-Dependent Formation and Degradation of Bone Matrix-Comparison of Two Cryogels.
3D-culture
bone cells
bone tissue engineering
cryogel
gelatin
matrix metabolism
platelet-rich plasma (PRP)
scaffold
Journal
Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056
Informations de publication
Date de publication:
05 Jun 2020
05 Jun 2020
Historique:
received:
15
05
2020
revised:
02
06
2020
accepted:
03
06
2020
entrez:
11
6
2020
pubmed:
11
6
2020
medline:
11
6
2020
Statut:
epublish
Résumé
Cryogels represent ideal carriers for bone tissue engineering. We recently described the osteogenic potential of cryogels with different protein additives, e.g., platelet-rich plasma (PRP). However, these scaffolds raised concerns as different toxic substances are required for their preparation. Therefore, we developed another gelatin (GEL)-based cryogel. This study aimed to compare the two scaffolds regarding their physical characteristics and their influence on osteogenic and osteoclastic cells. Compared to the PRP scaffolds, GEL scaffolds had both larger pores and thicker walls, resulting in a lower connective density. PRP scaffolds, with crystalized calcium phosphates on the surface, were significantly stiffer but less mineralized than GEL scaffolds with hydroxyapatite incorporated within the matrix. The GEL scaffolds favored adherence and proliferation of the osteogenic SCP-1 and SaOS-2 cells. Macrophage colony-stimulating factor (M-CSF) and osteoprotegerin (OPG) levels seemed to be induced by GEL scaffolds. Levels of other osteoblast and osteoclast markers were comparable between the two scaffolds. After 14 days, mineral content and stiffness of the cryogels were increased by SCP-1 and SaOS-2 cells, especially of PRP scaffolds. THP-1 cell-derived osteoclastic cells only reduced mineral content and stiffness of PRP cryogels. In summary, both scaffolds present powerful advantages; however, the possibility to altered mineral content and stiffness may be decisive when it comes to using PRP or GEL scaffolds for bone tissue engineering.
Identifiants
pubmed: 32517006
pii: bioengineering7020052
doi: 10.3390/bioengineering7020052
pmc: PMC7378764
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministerium für Ländlichen Raum und Verbraucherschutz Baden-Württemberg
ID : 14-(34)-8402.43
Organisme : Philip Morris International
ID : -
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