Quality of DNA extracted from formalin-fixed, paraffin-embedded canine tissues.
DNA extraction
canine
formalin fixation
genetics
genome
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
Titre abrégé: J Vet Diagn Invest
Pays: United States
ID NLM: 9011490
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
pubmed:
11
6
2020
medline:
20
11
2020
entrez:
11
6
2020
Statut:
ppublish
Résumé
Veterinary pathology tissue banks are valuable resources for genetic studies. However, limited data exist as to whether quality DNA can be extracted from these tissues for use in canine genotyping studies. We extracted DNA from 44 formalin-fixed, paraffin-embedded (FFPE) tissue blocks from dogs; 9 of these dogs had DNA available from whole blood samples that had been banked. We genotyped DNA from 30 of 44 tissue blocks and 9 whole blood samples on the Illumina CanineHD BeadChip; DNA quality was insufficient in 14 of 44 samples from tissue blocks. There was significant correlation between the 260/280 ratio and single-nucleotide variation (SNV) call rate (
Identifiants
pubmed: 32517550
doi: 10.1177/1040638720929637
pmc: PMC7438656
doi:
Substances chimiques
Formaldehyde
1HG84L3525
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
556-559Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR001860
Pays : United States
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