TRPM5 Negatively Regulates Calcium-Dependent Responses in Lipopolysaccharide-Stimulated B Lymphocytes.

Animals B-Lymphocytes / drug effects Calcium / metabolism HEK293 Cells Humans Lipopolysaccharides / pharmacology Lymphocyte Activation Mice Mice, Inbred C57BL RAW 264.7 Cells Signal Transduction TRPM Cation Channels / metabolism
B lymphocytes Ca(2+) signaling IL-6 TRPM5 endotoxic shock

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
09 06 2020
Historique:
received: 02 11 2019
revised: 16 03 2020
accepted: 20 05 2020
entrez: 11 6 2020
pubmed: 11 6 2020
medline: 20 5 2021
Statut: ppublish

Résumé

B cells produce high amounts of cytokines and immunoglobulins in response to lipopolysaccharide (LPS) stimulation. Calcium signaling cascades are critically involved in cytokine production of T cells, and the cytosolic calcium concentration is regulated by calcium-activated monovalent cation channels (CAMs). Calcium signaling is also implicated in B cell activation; however, its involvement in the cytokine production of LPS-stimulated B cells remains less well characterized. Here, we show that the transient receptor potential melastatin 5 channel (TRPM5), which is one of the CAMs, negatively modulates calcium signaling, thereby regulating LPS-induced proliferative and inflammatory responses by B cells. LPS-stimulated B cells of Trpm5-deficient mice exhibit an increased cytosolic calcium concentration, leading to enhanced proliferation and the production of the inflammatory cytokines interleukin-6 and CXCL10. Furthermore, Trpm5-deficient mice show an exacerbation of endotoxic shock with high mortality. Our findings demonstrate the importance of TRPM5-dependent regulatory mechanisms in LPS-induced calcium signaling of splenic B cells.

Identifiants

DOI: 10.1016/j.celrep.2020.107755 PMID: 32521253
pubmed: 32521253
pii: S2211-1247(20)30735-X
doi: 10.1016/j.celrep.2020.107755
pii:
doi:

Substances chimiques

Lipopolysaccharides 0
TRPM Cation Channels 0
TRPM5 protein, human 0
Trpm5 protein, mouse 0
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

107755

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

Auteurs

Taiki Sakaguchi (T)

Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Japan; WPI Immunology Frontier Research Center, Osaka University, Suita, Japan; Department of Cardiovascular Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.

Ryu Okumura (R)

Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Japan; WPI Immunology Frontier Research Center, Osaka University, Suita, Japan.

Chisato Ono (C)

Division of Immunology and Genome Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Daisuke Okuzaki (D)

Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Japan.

Takafumi Kawai (T)

Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Japan.

Yoshifumi Okochi (Y)

Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Japan.

Natsuko Tanimura (N)

Department of Pharmacology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan.

Mari Murakami (M)

Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Japan; WPI Immunology Frontier Research Center, Osaka University, Suita, Japan.

Hisako Kayama (H)

Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Japan; WPI Immunology Frontier Research Center, Osaka University, Suita, Japan; Institute for Advanced Co-Creation Studies, Osaka University, Suita, Japan.

Eiji Umemoto (E)

Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Japan; WPI Immunology Frontier Research Center, Osaka University, Suita, Japan.

Hidetaka Kioka (H)

Department of Cardiovascular Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.

Tomohito Ohtani (T)

Department of Cardiovascular Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.

Yasushi Sakata (Y)

Department of Cardiovascular Medicine, Graduate School of Medicine, Osaka University, Suita, Japan.

Kensuke Miyake (K)

Division of Innate Immunity, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Yasushi Okamura (Y)

Laboratory of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Japan.

Yoshihiro Baba (Y)

Division of Immunology and Genome Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Kiyoshi Takeda (K)

Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Suita, Japan; WPI Immunology Frontier Research Center, Osaka University, Suita, Japan; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Japan. Electronic address: ktakeda@ongene.med.osaka-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes B › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines bactériennes › Endotoxines › Lipopolysaccharides › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Phénomènes du système immunitaire › Activation des lymphocytes › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Cellules › Cellules RAW 264.7 › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › TRPM5 Negatively Regulates Calcium-Dependent...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux cationiques TRP › Canaux cationiques TRPM › TRPM5 Negatively Regulates Calcium-Dependent...