Notch-Mediated Generation of Monocyte-Derived Langerhans Cells: Phenotype and Function.


Journal

The Journal of investigative dermatology
ISSN: 1523-1747
Titre abrégé: J Invest Dermatol
Pays: United States
ID NLM: 0426720

Informations de publication

Date de publication:
01 2021
Historique:
received: 27 11 2019
revised: 01 05 2020
accepted: 04 05 2020
pubmed: 12 6 2020
medline: 7 9 2021
entrez: 12 6 2020
Statut: ppublish

Résumé

Langerhans cells (LCs) in the skin are a first line of defense against pathogens but also play an essential role in skin homeostasis. Their exclusive expression of the C-type lectin receptor Langerin makes them prominent candidates for immunotherapy. For vaccine testing, an easily accessible cell platform would be desirable as an alternative to the time-consuming purification of LCs from human skin. Here, we present such a model and demonstrate that monocytes in the presence of GM-CSF, TGF-β1, and the Notch ligand DLL4 differentiate within 3 days into CD1a

Identifiants

pubmed: 32522485
pii: S0022-202X(20)31663-8
doi: 10.1016/j.jid.2020.05.098
pmc: PMC7758629
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

84-94.e6

Subventions

Organisme : Austrian Science Fund FWF
ID : P 29919
Pays : Austria

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Lydia Bellmann (L)

Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.

Claudia Zelle-Rieser (C)

Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.

Paul Milne (P)

Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.

Anastasia Resteu (A)

Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.

Christoph H Tripp (CH)

Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.

Natascha Hermann-Kleiter (N)

Institute of Cell Genetics, Department for Genetics and Pharmacology, Medical University of Innsbruck, Innsbruck, Austria.

Viktoria Zaderer (V)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.

Doris Wilflingseder (D)

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.

Paul Hörtnagl (P)

Central Institute for Blood Transfusion and Immunological Department, Medical University of Innsbruck, Innsbruck, Austria.

Maria Theochari (M)

Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.

Jessica Schulze (J)

Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.

Mareike Rentzsch (M)

Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.

Barbara Del Frari (B)

Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Innsbruck, Austria.

Matthew Collin (M)

Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.

Christoph Rademacher (C)

Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.

Nikolaus Romani (N)

Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria.

Patrizia Stoitzner (P)

Department of Dermatology, Venereology & Allergology, Medical University of Innsbruck, Innsbruck, Austria. Electronic address: patrizia.stoitzner@i-med.ac.at.

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Classifications MeSH