Graphene Oxide Nano-Concentrators Selectively Modulate RNA Trapping According to Metal Cations in Solution.
diagnostics
graphene
miRNA
nanotechnology
nucleic acid
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
02
2020
accepted:
14
04
2020
entrez:
12
6
2020
pubmed:
12
6
2020
medline:
12
6
2020
Statut:
epublish
Résumé
With recent advances in nanotechnology, graphene nanomaterials are being translated to applications in the fields of biosensing, medicine, and diagnostics, with unprecedented power. Graphene is a carbon allotrope derived from graphite exfoliation made of an extremely thin honeycomb of sp2 hybridized carbons. In comparison with the bulk materials, graphene and its water-soluble derivative graphene oxide have a smaller size suitable for diagnostic platform miniaturization as well as high surface area and consequently loading of a large number of biological probes. In this work, we propose a nanotechnological method for concentrating total RNA solution and/or enriching small RNA molecules. To this aim, we exploited the unique trapping effects of GO nanoflakes in the presence of divalent cations (i.e., calcium and magnesium) that make it flocculate and precipitate, forming complex meshes that are positively charged. Here, we demonstrated that GO traps can concentrate nucleic acids in the presence of divalent cations and that small RNAs can be selectively released from GO-magnesium traps. GO nano-concentrators will allow better analytical performance with samples available in small amounts and will increase the sensitivity of sequencing platforms by short RNA selection.
Identifiants
pubmed: 32523936
doi: 10.3389/fbioe.2020.00421
pmc: PMC7261913
doi:
Types de publication
Journal Article
Langues
eng
Pagination
421Informations de copyright
Copyright © 2020 Palmieri, Di Pietro, Perini, Barba, Parolini, De Spirito, Lattanzi and Papi.
Références
Sci Rep. 2017 Nov 28;7(1):16510
pubmed: 29184216
Sci Data. 2015 Nov 10;2:150063
pubmed: 26594381
Int J Oncol. 2018 Oct;53(4):1395-1434
pubmed: 30085333
Biosens Bioelectron. 2018 Jan 15;99:612-624
pubmed: 28837925
Interface Focus. 2018 Jun 6;8(3):20170059
pubmed: 29696091
Biomark Med. 2017 Jan;11(1):69-86
pubmed: 27917642
RNA. 2004 Mar;10(3):335-43
pubmed: 14970378
Langmuir. 2013 Dec 10;29(49):15174-81
pubmed: 24261814
J Biomed Biotechnol. 2012;2012:813894
pubmed: 23097599
Trends Biochem Sci. 2019 May;44(5):433-452
pubmed: 30686572
PLoS One. 2018 Feb 23;13(2):e0191966
pubmed: 29474379
Nanotechnology. 2013 Jul 5;24(26):265703
pubmed: 23732310
Talanta. 2019 May 1;196:329-336
pubmed: 30683372
Int J Mol Sci. 2018 Oct 26;19(11):
pubmed: 30373116
Chem Commun (Camb). 2019 Apr 4;55(29):4186-4189
pubmed: 30892320
Chem Soc Rev. 2014 Aug 7;43(15):5288-301
pubmed: 24789533
Proteomics Clin Appl. 2015 Feb;9(1-2):169-86
pubmed: 25488355
Langmuir. 2011 Mar 15;27(6):2731-8
pubmed: 21302946
Bone. 2018 Jul;112:58-70
pubmed: 29674126
Environ Sci Technol. 2016 Oct 18;50(20):11066-11075
pubmed: 27662468
PLoS One. 2018 Dec 7;13(12):e0208580
pubmed: 30532175
Sci Rep. 2017 Aug 25;7(1):9538
pubmed: 28842714
Sci Rep. 2016 Jan 20;6:19529
pubmed: 26787294
Adv Drug Deliv Rev. 2016 Oct 1;105(Pt B):176-189
pubmed: 27129441
RNA. 2011 Feb;17(2):291-7
pubmed: 21173199
PLoS One. 2014 May 05;9(5):e94839
pubmed: 24797360
Nanotechnology. 2017 Apr 18;28(15):152001
pubmed: 28303804
Anal Chem. 2017 Oct 3;89(19):10137-10140
pubmed: 28933157