A Schwann Cell-Neuron Coculture System to Study Neuron-Glia Interaction During Axon Degeneration.
Animals
Axons
/ physiology
Axotomy
Cell Separation
/ methods
Cells, Cultured
Coculture Techniques
/ instrumentation
Ganglia, Spinal
/ cytology
Immunomagnetic Separation
/ methods
Laminin
Mice
Microscopy, Fluorescence
/ methods
Nerve Growth Factor
/ pharmacology
Polylysine
Rats
Rats, Sprague-Dawley
Schwann Cells
/ cytology
Sciatic Nerve
/ cytology
Sensory Receptor Cells
/ cytology
Wallerian Degeneration
/ physiopathology
Axon
Axon degeneration
Coculture
In vitro injury
Neuro–glia interaction
Schwann cell
Spot culture
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
12
6
2020
pubmed:
12
6
2020
medline:
17
3
2021
Statut:
ppublish
Résumé
Autonomous mechanisms of axon degeneration are frequently studied in vitro by mechanical axon injury of isolated sensory neurons. This has led to major advances in understanding the molecular pathways governing axon degeneration. However, this approach does not pay attention to potential glial mechanisms for the regulation of axon death. Here, I describe a straightforward protocol to seed purified rat Schwann cells on neuronal cultures in order to study the interaction between axons and these glia during axon degeneration.
Identifiants
pubmed: 32524475
doi: 10.1007/978-1-0716-0585-1_8
doi:
Substances chimiques
Laminin
0
Polylysine
25104-18-1
Nerve Growth Factor
9061-61-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM