Localisation of cannabinoid and cannabinoid-related receptors in the equine dorsal root ganglia.
5-HT1aR
CB1
CB2
PPARα
TRPA1
horse
Journal
Equine veterinary journal
ISSN: 2042-3306
Titre abrégé: Equine Vet J
Pays: United States
ID NLM: 0173320
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
29
04
2020
received:
27
01
2020
accepted:
25
05
2020
pubmed:
12
6
2020
medline:
13
4
2021
entrez:
12
6
2020
Statut:
ppublish
Résumé
Growing evidence recognises cannabinoid receptors as potential therapeutic targets for pain. Consequently, there is increasing interest in developing cannabinoid receptor agonists for treating pain. As a general rule, to better understand the actions of a drug, it would be of extreme importance to know the cellular distribution of its specific receptors. The localisation of cannabinoid receptors in the dorsal root ganglia of the horse has not yet been investigated. To localise the cellular distribution of canonical and putative cannabinoid receptors in the equine cervical dorsal root ganglia. Qualitative and quantitative immunohistochemical study. Cervical (C6-C8) dorsal root ganglia were collected from six horses (1.5 years of age) at the slaughterhouse. The tissues were fixed and processed to obtain cryosections which were used to investigate the immunoreactivity of canonical cannabinoid receptors 1 (CB1R) and 2 (CB2R), and for three putative cannabinoid-related receptors: nuclear peroxisome proliferator-activated receptor alpha (PPARα), transient receptor potential ankyrin 1 (TRPA1) and serotonin 5-HT1a receptor (5-HT1aR). The neurons showed immunoreactivity for CB1R (100%), CB2R (80% ± 13%), PPARα (100%), TRPA1 (74% ± 10%) and 5-HT1aR (84% ± 6%). The neuronal satellite glial cells showed immunoreactivity for CB2R, PPARα, TRPA1 and 5-HT1aR. The low number of horses included in the study. This study highlighted the expression of cannabinoid receptors in the sensory neurons and glial cells of the dorsal root ganglia. These findings could be of particular relevance for future functional studies assessing the effects of cannabinoids in horses to manage pain.
Sections du résumé
BACKGROUND
BACKGROUND
Growing evidence recognises cannabinoid receptors as potential therapeutic targets for pain. Consequently, there is increasing interest in developing cannabinoid receptor agonists for treating pain. As a general rule, to better understand the actions of a drug, it would be of extreme importance to know the cellular distribution of its specific receptors. The localisation of cannabinoid receptors in the dorsal root ganglia of the horse has not yet been investigated.
OBJECTIVES
OBJECTIVE
To localise the cellular distribution of canonical and putative cannabinoid receptors in the equine cervical dorsal root ganglia.
STUDY DESIGN
METHODS
Qualitative and quantitative immunohistochemical study.
METHODS
METHODS
Cervical (C6-C8) dorsal root ganglia were collected from six horses (1.5 years of age) at the slaughterhouse. The tissues were fixed and processed to obtain cryosections which were used to investigate the immunoreactivity of canonical cannabinoid receptors 1 (CB1R) and 2 (CB2R), and for three putative cannabinoid-related receptors: nuclear peroxisome proliferator-activated receptor alpha (PPARα), transient receptor potential ankyrin 1 (TRPA1) and serotonin 5-HT1a receptor (5-HT1aR).
RESULTS
RESULTS
The neurons showed immunoreactivity for CB1R (100%), CB2R (80% ± 13%), PPARα (100%), TRPA1 (74% ± 10%) and 5-HT1aR (84% ± 6%). The neuronal satellite glial cells showed immunoreactivity for CB2R, PPARα, TRPA1 and 5-HT1aR.
MAIN LIMITATIONS
CONCLUSIONS
The low number of horses included in the study.
CONCLUSIONS
CONCLUSIONS
This study highlighted the expression of cannabinoid receptors in the sensory neurons and glial cells of the dorsal root ganglia. These findings could be of particular relevance for future functional studies assessing the effects of cannabinoids in horses to manage pain.
Substances chimiques
Cannabinoids
0
Receptors, Cannabinoid
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
549-557Subventions
Organisme : Formula Swiss AG, Switzerland
Informations de copyright
© 2020 EVJ Ltd.
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