Green tea extract solutions can control bacterial biofilms formed on space maintainers.
Anti-Bacterial Agents
/ pharmacology
Antioxidants
/ pharmacology
Bacterial Load
/ drug effects
Biofilms
/ drug effects
Child
Chlorhexidine
/ pharmacology
Dental Caries
/ drug therapy
Humans
Methanol
Microbial Sensitivity Tests
Mouth
Orthodontic Brackets
/ microbiology
Plant Extracts
/ chemistry
Solvents
/ chemistry
Streptococcal Infections
/ drug therapy
Streptococcus mutans
/ drug effects
Tea
/ chemistry
Turkey
Biofilm
green tea
oral microorganisms
spacemaintainers
Journal
Nigerian journal of clinical practice
ISSN: 1119-3077
Titre abrégé: Niger J Clin Pract
Pays: India
ID NLM: 101150032
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
entrez:
12
6
2020
pubmed:
12
6
2020
medline:
18
7
2020
Statut:
ppublish
Résumé
Microorganisms in the mouth are protected from negative environmental conditions by forming biofilms; however, the use of anti-plaque agents in children is not preferred due to toxic side effects. Green tea has been reported to have anti-microbial and anti-dental caries properties. The aim of this study was to assess the ability of green tea extract to prevent the formation of biofilm on the teeth of children using space maintainers. Bacteria were isolated from samples obtained from children aged between 8 and 10 years. The micro-titer plate method and Congo red agar were used to assay biofilm formation. Green tea leaves were obtained from Rize, Turkey. Methanol, hexane and distilled water were used for preparing the extracts. The effects of green tea extract and chlorhexidine on biofilm formation were examined using scanning electron microscopy. Presence of S. mutans 3,3, S. anginosus 2.1.b, S. dysgalactie 6.1.4.1, and E. faecium 10.2. was measured in the biofilm samples. The extracts showed a bacteriostatic effect on the test bacteria, and among the green tea extracts, the methanol extract was found to exhibit the highest efficacy against biofilm formation by S. mutans 3.3. Green tea extract showed good efficacy in controlling bacterial growth, and is recommended as a better-tasting alternative for daily oral hygiene due to a lack of known side effects.
Sections du résumé
BACKGROUND
BACKGROUND
Microorganisms in the mouth are protected from negative environmental conditions by forming biofilms; however, the use of anti-plaque agents in children is not preferred due to toxic side effects. Green tea has been reported to have anti-microbial and anti-dental caries properties.
AIMS
OBJECTIVE
The aim of this study was to assess the ability of green tea extract to prevent the formation of biofilm on the teeth of children using space maintainers.
METHODS
METHODS
Bacteria were isolated from samples obtained from children aged between 8 and 10 years. The micro-titer plate method and Congo red agar were used to assay biofilm formation. Green tea leaves were obtained from Rize, Turkey. Methanol, hexane and distilled water were used for preparing the extracts. The effects of green tea extract and chlorhexidine on biofilm formation were examined using scanning electron microscopy.
RESULTS
RESULTS
Presence of S. mutans 3,3, S. anginosus 2.1.b, S. dysgalactie 6.1.4.1, and E. faecium 10.2. was measured in the biofilm samples. The extracts showed a bacteriostatic effect on the test bacteria, and among the green tea extracts, the methanol extract was found to exhibit the highest efficacy against biofilm formation by S. mutans 3.3.
CONCLUSION
CONCLUSIONS
Green tea extract showed good efficacy in controlling bacterial growth, and is recommended as a better-tasting alternative for daily oral hygiene due to a lack of known side effects.
Identifiants
pubmed: 32525112
pii: NigerJClinPract_2020_23_6_783_286431
doi: 10.4103/njcp.njcp_246_19
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antioxidants
0
Plant Extracts
0
Solvents
0
Tea
0
Chlorhexidine
R4KO0DY52L
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
783-791Déclaration de conflit d'intérêts
None