Adaptive Enrichment of a Thermophilic Bacterial Isolate for Enhanced Enzymatic Activity.
Geobacillus
adaptive laboratory evolution
laccase
lignocellulosic
thermophile
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
09 Jun 2020
09 Jun 2020
Historique:
received:
07
05
2020
revised:
03
06
2020
accepted:
04
06
2020
entrez:
13
6
2020
pubmed:
13
6
2020
medline:
13
6
2020
Statut:
epublish
Résumé
The mimicking of evolution on a laboratory timescale to enhance biocatalyst specificity, substrate utilization activity, and/or product formation, is an effective and well-established approach that does not involve genetic engineering or regulatory details of the microorganism. The present work employed an evolutionary adaptive approach to improve the lignocellulose deconstruction capabilities of the strain by inducing the expression of laccase, a multicopper oxidase, in
Identifiants
pubmed: 32526936
pii: microorganisms8060871
doi: 10.3390/microorganisms8060871
pmc: PMC7355623
pii:
doi:
Types de publication
Journal Article
Langues
eng
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