Effect of life stage and pesticide exposure on the gut microbiota of Aedes albopictus and Culex pipiens L.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 06 2020
11 06 2020
Historique:
received:
03
12
2019
accepted:
11
05
2020
entrez:
13
6
2020
pubmed:
13
6
2020
medline:
1
12
2020
Statut:
epublish
Résumé
Pesticides commonly contaminate the aquatic environments inhabited by mosquito juveniles. However, their role in shaping the mosquito microbiota is not well understood. We hypothesized that environmentally relevant concentrations of atrazine, permethrin and malathion will mediate a shift in the mosquito gut bacterial community structure due to their toxic effect on the aquatic bacterial communities, and reduce mosquito gut bacterial diversity by enriching pesticide-degrading bacterial communities over susceptible taxa. Illumina MiSeq sequencing of the V3-V4 hypervariable regions of the 16 S rRNA gene was used to characterize the microbial communities of larval and adult stages of the two mosquito species and the water samples from microcosms treated with each of the pesticides, separately. Bacterial community composition differed by sample type (larval stage vs. adult stage) and water sampling date (day 3 vs. day 7), but not by pesticide treatment. In larval stages, bacterial OTU richness was highest in samples exposed to malathion, intermediate in permethrin, and lowest in controls. Bacterial richness was significantly higher in larval stages compared to adult stages for all treatments. This study provides a primer for future studies evaluating mosquito microbial responses to exposures to chemical pesticides and the possible implications for mosquito ecology.
Identifiants
pubmed: 32528116
doi: 10.1038/s41598-020-66452-5
pii: 10.1038/s41598-020-66452-5
pmc: PMC7289809
doi:
Substances chimiques
Pesticides
0
Permethrin
509F88P9SZ
Atrazine
QJA9M5H4IM
Malathion
U5N7SU872W
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
9489Références
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