Cell-Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium.
RNA-seq
Ribotag
hair cell
heat shock
supporting cell
utricle
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2020
2020
Historique:
received:
10
01
2020
accepted:
15
04
2020
entrez:
13
6
2020
pubmed:
13
6
2020
medline:
13
6
2020
Statut:
epublish
Résumé
Sensory epithelia of the inner ear contain mechanosensory hair cells (HCs) and glia-like supporting cells (SCs), both of which are required for hearing and balance functions. Each of these cell types has unique responses to ototoxic and cytoprotective stimuli. Non-lethal heat stress in the mammalian utricle induces heat shock proteins (HSPs) and protects against ototoxic drug-induced hair cell death. Induction of HSPs in the utricle demonstrates cell-type specificity at the protein level, with HSP70 induction occurring primarily in SCs, while HSP32 (also known as heme oxygenase 1, HMOX1) is induced primarily in resident macrophages. Neither of these HSPs are robustly induced in HCs, suggesting that HCs may have little capacity for induction of stress-induced protective responses. To determine the transcriptional responses to heat shock of these different cell types, we performed cell-type-specific transcriptional profiling using the RiboTag method, which allows for immunoprecipitation (IP) of actively translating mRNAs from specific cell types. RNA-Seq differential gene expression analyses demonstrated that the RiboTag method identified known cell type-specific markers as well as new markers for HCs and SCs. Gene expression differences suggest that HCs and SCs exhibit differential transcriptional heat shock responses. The chaperonin family member
Identifiants
pubmed: 32528249
doi: 10.3389/fncel.2020.00123
pmc: PMC7247426
doi:
Types de publication
Journal Article
Langues
eng
Pagination
123Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC000086
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC013817
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007445
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA DC000079
Pays : United States
Informations de copyright
Copyright © 2020 Sadler, Ryals, May, Martin, Welsh, Boger, Morell, Hertzano and Cunningham.
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