Immune Cells in the Placental Villi Contribute to Intra-amniotic Inflammation.
Animals
Biomarkers
/ metabolism
Chorioamnionitis
/ chemically induced
Chorionic Villi
/ drug effects
Decidua
/ drug effects
Disease Models, Animal
Female
Immunophenotyping
Leukocytes
/ drug effects
Lipopolysaccharides
Lymphocyte Activation
Macaca mulatta
Pregnancy
Signal Transduction
Tumor Necrosis Factor Inhibitors
/ pharmacology
Tumor Necrosis Factor-alpha
/ metabolism
CyTOF
choriodecidua
immune
intra-amniotic inflammation
placental villi
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
31
01
2020
accepted:
15
04
2020
entrez:
13
6
2020
pubmed:
13
6
2020
medline:
30
3
2021
Statut:
epublish
Résumé
Intra-amniotic (IA) inflammation is associated with significant morbidities for both the mother and the fetus. Prior studies have illustrated many of the effects of IA inflammation on the uterine lining (decidua) and membranous layers of the placenta at the fetal-maternal interface. However, much less is known about the immunological response occurring within the villous placenta. Using a rhesus macaque model of lipopolysaccharide (LPS)-induced IA inflammation, we showed that pregnancy-matched choriodecidua and villi have distinct immunological profiles in rhesus pregnancies. In the choriodecidua, we show that the abundance of neutrophils, multiple populations of antigen-presenting cells, and two populations of natural killer (NK) cells changes with prenatal IA LPS exposure. In contrast, in immune cells within the villous placenta we observed alterations in the abundance of B cells, monocytes, and CD8 T cells. Prior work has illustrated that IA inflammation leads to an increase in tumor necrosis factor alpha (TNFα) at the fetal-maternal interface. In this study, pretreatment with a TNFα blockade partially reversed inflammation in the placental villi. Furthermore, we report that immune cells in the villous placenta sensed LPS during our experimental window, and subsequently activated T cells to produce proinflammatory cytokines. Moreover, this study is the first report of memory T cells in third-trimester non-human primate placental villi and provides evidence that manipulation of immune cells in the villi at the fetal-maternal interface should be considered as a potential therapeutic target for IA inflammation.
Identifiants
pubmed: 32528468
doi: 10.3389/fimmu.2020.00866
pmc: PMC7256198
doi:
Substances chimiques
Biomarkers
0
Lipopolysaccharides
0
Tumor Necrosis Factor Inhibitors
0
Tumor Necrosis Factor-alpha
0
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
866Subventions
Organisme : NICHD NIH HHS
ID : R01 HD098389
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD090856
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI089443
Pays : United States
Organisme : NIEHS NIH HHS
ID : U01 ES029234
Pays : United States
Informations de copyright
Copyright © 2020 Toothaker, Presicce, Cappelletti, Stras, McCourt, Chougnet, Kallapur and Konnikova.
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