Development of a UHPLC-MS/MS method for the determination of 84 pharmaceuticals and drugs of abuse in human liver.

DOA Forensic toxicology Liver Post-mortem QuEChERS

Journal

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554

Informations de publication

Date de publication:
26 May 2020
Historique:
received: 02 04 2020
revised: 22 05 2020
accepted: 24 05 2020
pubmed: 13 6 2020
medline: 13 6 2020
entrez: 13 6 2020
Statut: aheadofprint

Résumé

Analysis of post-mortem liver for toxicological reasons is a considerable option when blood is unavailable. The development of analytical methods for tissue specimens is not as straightforward as for biological fluids as tissue presents challenges to the analytical chemist. The present study reports the development of a UHPLC-MS/MS method for the detection and quantification of 84 drugs and pharmaceuticals in human liver. The selected target drugs include pharmaceutical drugs and drugs of abuse. Sample preparation was studied using QuEChERS and different ratios of solvent volume and sample mass. Best results were attained by homogenizing 1 g of liver with acetonitrile K

Identifiants

DOI: 10.1016/j.jchromb.2020.122192 PMID: 32531702
pubmed: 32531702
pii: S1570-0232(20)30494-3
doi: 10.1016/j.jchromb.2020.122192
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122192

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest All Authors declare no conflict of interest.

Auteurs

Amvrosios Orfanidis (A)

Department of Medicine, Laboratory of Forensic Medicine and Toxicology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; BIOMIC_AUTH, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research and Innovation, CIRI-AUTH B1.4, Aristotle University of Thessaloniki, Thessaloniki-Thermi road, Greece.

Helen Gika (H)

Department of Medicine, Laboratory of Forensic Medicine and Toxicology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; BIOMIC_AUTH, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research and Innovation, CIRI-AUTH B1.4, Aristotle University of Thessaloniki, Thessaloniki-Thermi road, Greece. Electronic address: gkikae@auth.gr.

Georgios Theodoridis (G)

Department of Chemistry, Laboratory of Analytical Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; BIOMIC_AUTH, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research and Innovation, CIRI-AUTH B1.4, Aristotle University of Thessaloniki, Thessaloniki-Thermi road, Greece.

Orthodoxia Mastrogianni (O)

Laboratory of Forensic Service of Ministry of Justice of Thessaloniki, Palaia Symmahiki odos, 56334 Thessaloniki, Greece.

Nikolaos Raikos (N)

Department of Medicine, Laboratory of Forensic Medicine and Toxicology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; BIOMIC_AUTH, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research and Innovation, CIRI-AUTH B1.4, Aristotle University of Thessaloniki, Thessaloniki-Thermi road, Greece. Electronic address: raikos@auth.gr.

Classifications MeSH