A quantitative serum biomarker of circulating collagen X effectively correlates with endochondral fracture healing.
CXM assay
endochondral ossification
fracture healing
serum biomarker
type X collagen
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
03
02
2020
revised:
12
05
2020
accepted:
25
05
2020
pubmed:
14
6
2020
medline:
1
5
2021
entrez:
14
6
2020
Statut:
ppublish
Résumé
Currently, there are no standardized methods for quantitatively measuring fracture repair. Physicians rely on subjective physical examinations and qualitative evaluation of radiographs to detect mineralized tissue. Since most fractures heal indirectly through a cartilage intermediate, these tools are limited in their diagnostic utility of early repair. Prior to converting to the bone, cartilage undergoes hypertrophic maturation, characterized by the deposition of a provisional collagen X matrix. The objective of this study was to characterize the kinetics of a novel collagen X biomarker relative to other biological measurements of fracture healing using a murine model of endochondral fracture repair in which a closed, mid-shaft tibia fracture was created using the classic drop-weight technique. Serum was collected 5 to 42 days post-fracture in male and female mice and compared to uninjured controls (n = 8-12). Collagen X in the serum was quantified using a recently validated ELISA-based bioassay ("Cxm")
Substances chimiques
Biomarkers
0
Collagen Type X
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-62Informations de copyright
© 2020 Orthopaedic Research Society. Published by Wiley Periodicals LLC.
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