The Effect of Different Vitrification and Staining Protocols on the Visibility of the Nuclear Maturation Stage of Equine Oocytes.
Cryopreservation
Cryoprotectant
Hoechst 33342
Mare
Meiotic competence
Journal
Journal of equine veterinary science
ISSN: 0737-0806
Titre abrégé: J Equine Vet Sci
Pays: United States
ID NLM: 8216840
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
16
12
2019
revised:
01
04
2020
accepted:
02
04
2020
entrez:
15
6
2020
pubmed:
15
6
2020
medline:
25
2
2021
Statut:
ppublish
Résumé
In this study, we compared two staining protocols assessing the nuclear chromatin stage of equine oocytes after vitrification using permeable and nonpermeable cryoprotectants. Slaughterhouse-derived oocytes (n = 155) were obtained from a total of 32 mares and in vitro matured in M199 medium for 42 hours at 38.5°C in 5% CO
Identifiants
pubmed: 32534785
pii: S0737-0806(20)30112-X
doi: 10.1016/j.jevs.2020.103021
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103021Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.