A Simple Method to Determine the Fractions of Labile and Mineral-Bound Microelements in Bone Tissue by Atomic Absorption Spectrometry.
Apatite
Atomic absorption spectrometry
Bone tissue
Concentration
Localization
Microelements
Selective solubility
Journal
Biological trace element research
ISSN: 1559-0720
Titre abrégé: Biol Trace Elem Res
Pays: United States
ID NLM: 7911509
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
29
01
2020
accepted:
03
06
2020
pubmed:
15
6
2020
medline:
22
6
2021
entrez:
15
6
2020
Statut:
ppublish
Résumé
In this work a simple and inexpensive method to assess the concentration ratio of the labile and mineral-bound microelements of the bone tissue was developed. The approach is based on the separation of the components of bone tissue by their selective solubility with the subsequent determination of microelements with atomic absorption spectrometry. The total concentrations of Mg, Zn, Fe, Sr, Al, Cu, and Mn and the concentrations of these elements in aqueous solutions with pH 6.5, 10, and 12 after their ultrasonically activated interaction with the powder of dried bone were determined. Two quite different bone samples were analyzed: a cortical fragment of the femur of a mature healthy cow and the spongy part of a human femoral head affected by osteoporosis. Some common and individual features of the both type of bones in regard to the total concentrations and fractional distribution of microelements are discussed. The obtained concentrations of the "soluble" fractions of microelements were critically analyzed taking into account the possible reactions leading to new insoluble phases' formation in alkaline solutions. Based on the data obtained, the ability of elements to form labile fractions in the bone tissue could be arranged in the following descending series: Mg ≥ Zn > Al > Fe > Mn > Cu > Sr.
Identifiants
pubmed: 32535747
doi: 10.1007/s12011-020-02234-4
pii: 10.1007/s12011-020-02234-4
doi:
Substances chimiques
Minerals
0
Trace Elements
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
935-943Références
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