Identification and evaluation of cell- growth-inhibiting bDtBPP-analogue degradation products from phosphite antioxidants used in polyolefin bioprocessing materials.
Cytotoxicity evaluation
Extractables and leachables
Gamma sterilization
LC-HRMS screening
Polyolefin antioxidant degradation
Single-use systems
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
06
03
2020
accepted:
25
05
2020
revised:
15
05
2020
pubmed:
15
6
2020
medline:
9
2
2021
entrez:
15
6
2020
Statut:
ppublish
Résumé
The inhibiting effect of the secondary phosphite antioxidant degradation product bis(2,4-di-tert-butylphenyl)phosphate (bDtBPP) on cell growth is well-known. The present study describes structurally related compounds which are likely to be formed from similar widely used phosphite antioxidants used in materials for the manufacturing of single-use (SU) equipment. Two potential candidates of such compounds-3,3',5,5'-tetra-tert-butyl-2,2'-dihydroxybiphenylphosphate (TtBBP) and bis(p-nonylphenyl)phosphate (bNPP)-were identified by chromatography and mass spectrometry followed by synthesis and X-ray structure elucidation. Additionally, the formation of TtBBP was confirmed in an analytical degradation study and its migration from SU bioprocessing material was estimated. The cytotoxicity evaluation by means of cell culture spiking experiments and flow cytometry analysis revealed that' even if cell growth was inhibited by all the compounds to some extent, bDtBPP showed the most severe effect and stoods out from the other two degradants investigated. Graphical abstract.
Identifiants
pubmed: 32535801
doi: 10.1007/s00216-020-02736-z
pii: 10.1007/s00216-020-02736-z
doi:
Substances chimiques
Antioxidants
0
Phosphites
0
Polyenes
0
PL 732
83136-87-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4505-4518Références
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