Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy.
Esophageal cancer
collagen fiber
esophageal squamous cell carcinoma
nonlinear optical microscopy
second harmonic generation
two-photon excited florescence
Journal
Experimental biology and medicine (Maywood, N.J.)
ISSN: 1535-3699
Titre abrégé: Exp Biol Med (Maywood)
Pays: England
ID NLM: 100973463
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
pubmed:
17
6
2020
medline:
23
2
2021
entrez:
16
6
2020
Statut:
ppublish
Résumé
The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.
Identifiants
pubmed: 32536201
doi: 10.1177/1535370220934039
pmc: PMC7437372
doi:
Substances chimiques
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1213-1221Références
Surg Endosc. 2011 May;25(5):1425-30
pubmed: 21046158
Cancer Res. 2018 May 15;78(10):2503-2512
pubmed: 29535219
BMC Cancer. 2010 Mar 11;10:94
pubmed: 20222963
Dis Esophagus. 2007;20(2):141-50
pubmed: 17439598
Biomed Opt Express. 2013 Nov 15;4(12):2855-68
pubmed: 24409386
Scanning. 2018 Apr 17;2018:3697063
pubmed: 29849858
Opt Commun. 2008 Apr 1;281(7):1823-1832
pubmed: 19343083
Methods Cell Biol. 2014;123:531-46
pubmed: 24974046
Opt Lett. 2012 Sep 15;37(18):3837-9
pubmed: 23041876
Biomed Opt Express. 2012 Jan 1;3(1):1-15
pubmed: 22254163
Scanning. 2015 Mar-Apr;37(2):152-7
pubmed: 25675898
BMC Cancer. 2019 Apr 2;19(1):295
pubmed: 30940105
Eur J Gastroenterol Hepatol. 2010 Jun;22(6):669-78
pubmed: 19474750
Photochem Photobiol. 2003 May;77(5):550-5
pubmed: 12812299
World J Gastroenterol. 2015 Apr 14;21(14):4210-5
pubmed: 25892870
Biophys J. 2004 Jun;86(6):3914-22
pubmed: 15189888
Biophys J. 2005 Feb;88(2):1377-86
pubmed: 15533922
Lasers Med Sci. 2016 Sep;31(7):1285-91
pubmed: 27299572
Br J Cancer. 2009 Sep 1;101(5):855-9
pubmed: 19672254
Head Neck. 2016 Apr;38 Suppl 1:E2343-9
pubmed: 26595056
Oncol Lett. 2016 Jun;11(6):3785-3789
pubmed: 27313695
J Struct Biol. 2010 Jul;171(1):88-94
pubmed: 20206272
Oncotarget. 2017 Nov 3;8(63):107323-107333
pubmed: 29291032
Proc SPIE Int Soc Opt Eng. 2010 Jan 24;7569:756918
pubmed: 20445820
Cancer Res. 2014 May 15;74(10):2688-97
pubmed: 24686168
Nat Protoc. 2012 Mar 08;7(4):654-69
pubmed: 22402635
Opt Express. 2007 Sep 17;15(19):12286-95
pubmed: 19547597
J Biomed Opt. 2008 Nov-Dec;13(6):064010
pubmed: 19123657
J Biomed Opt. 2007 Jul-Aug;12(4):044002
pubmed: 17867806
Biomed Opt Express. 2017 Jun 21;8(7):3360-3368
pubmed: 28717572
Opt Express. 2007 Sep 17;15(19):12005-10
pubmed: 19547564
J Natl Compr Canc Netw. 2019 Jul 1;17(7):855-883
pubmed: 31319389
Eur J Cancer Prev. 2017 Mar;26(2):107-118
pubmed: 27014938
Biophys J. 2007 Aug 15;93(4):1312-20
pubmed: 17449666
J Biomed Opt. 2014 Jan;19(1):16007
pubmed: 24407500
World J Gastrointest Endosc. 2009 Oct 15;1(1):45-50
pubmed: 21160650
Scanning. 2011 Jan-Feb;33(1):53-6
pubmed: 21328394
Ann Biomed Eng. 2012 Feb;40(2):277-91
pubmed: 22258888
Head Neck. 2019 Jan;41(1):116-121
pubmed: 30548511
J Biomed Opt. 2011 Apr;16(4):040502
pubmed: 21529064
J Biomed Opt. 2009 Jan-Feb;14(1):010504
pubmed: 19256686
J Microsc. 2014 Jan;253(1):79-82
pubmed: 24236445
Arch Pathol Lab Med. 2014 Feb;138(2):204-12
pubmed: 24476518
Scanning. 2015 Jan-Feb;37(1):17-22
pubmed: 25384355
Ann Cardiothorac Surg. 2017 Mar;6(2):99-109
pubmed: 28446998
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11014-9
pubmed: 12177437
J Biomed Opt. 2009 Mar-Apr;14(2):020503
pubmed: 19405709
Scanning. 2013 Nov-Dec;35(6):387-91
pubmed: 23427072
Lasers Med Sci. 2018 May;33(4):871-882
pubmed: 29411176