On the applicability of the Tubulin-Based Polymorphism (TBP) genotyping method: a comprehensive guide illustrated through the application on different genetic resources in the legume family.
Genotyping
Intron Length Polymorphism
Legumes
TBP (Tubulin Based-Polymorphism)
β-Tubulins
Journal
Plant methods
ISSN: 1746-4811
Titre abrégé: Plant Methods
Pays: England
ID NLM: 101245798
Informations de publication
Date de publication:
2020
2020
Historique:
received:
21
11
2019
accepted:
30
05
2020
entrez:
16
6
2020
pubmed:
17
6
2020
medline:
17
6
2020
Statut:
epublish
Résumé
Plant discrimination is of relevance for taxonomic, evolutionary, breeding and nutritional studies. To this purpose, evidence is reported to demonstrate TBP (Tubulin-Based-Polymorphism) as a DNA-based method suitable for assessing plant diversity. Exploiting one of the most valuable features of TBP, that is the convenient and immediate application of the assay to groups of individuals that may belong to different taxa, we show that the TBP method can successfully discriminate different agricultural species and their crop wild relatives within the Papilionoideae subfamily. Detection of intraspecific variability is demonstrated by the genotyping of 27 different accessions of These data illustrate TBP as a useful and versatile tool for plant genotyping. Since its potential has not yet been fully appreciated by the scientific community, we carefully report all the experimental details of a successful TBP protocol, while describing different applications, so that the method can be replicated in other laboratories.
Sections du résumé
BACKGROUND
BACKGROUND
Plant discrimination is of relevance for taxonomic, evolutionary, breeding and nutritional studies. To this purpose, evidence is reported to demonstrate TBP (Tubulin-Based-Polymorphism) as a DNA-based method suitable for assessing plant diversity.
RESULTS
RESULTS
Exploiting one of the most valuable features of TBP, that is the convenient and immediate application of the assay to groups of individuals that may belong to different taxa, we show that the TBP method can successfully discriminate different agricultural species and their crop wild relatives within the Papilionoideae subfamily. Detection of intraspecific variability is demonstrated by the genotyping of 27 different accessions of
CONCLUSIONS
CONCLUSIONS
These data illustrate TBP as a useful and versatile tool for plant genotyping. Since its potential has not yet been fully appreciated by the scientific community, we carefully report all the experimental details of a successful TBP protocol, while describing different applications, so that the method can be replicated in other laboratories.
Identifiants
pubmed: 32536963
doi: 10.1186/s13007-020-00627-z
pii: 627
pmc: PMC7291473
doi:
Types de publication
Journal Article
Langues
eng
Pagination
86Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
Références
BMC Evol Biol. 2012 Dec 25;12:250
pubmed: 23267563
Theor Appl Genet. 2010 Sep;121(5):829-43
pubmed: 20490446
Genome. 2004 Apr;47(2):281-91
pubmed: 15060580
Anal Bioanal Chem. 2016 Nov;408(29):8299-8316
pubmed: 27695983
Am J Bot. 2009 Jun;96(6):1177-89
pubmed: 21628268
Mol Biol Evol. 1987 Jul;4(4):406-25
pubmed: 3447015
Brief Funct Genomics. 2018 May 1;17(3):198-206
pubmed: 29688255
BMC Biol. 2008 Dec 17;6:55
pubmed: 19091055
J Food Drug Anal. 2017 Apr;25(2):260-274
pubmed: 28911667
Ann Bot. 2014 May;113(6):895-908
pubmed: 24623333
Sci Rep. 2017 Apr 3;7(1):528
pubmed: 28373664
PLoS Biol. 2004 Oct;2(10):e312
pubmed: 15455034
PLoS One. 2011;6(5):e19254
pubmed: 21637336
Nat Commun. 2014 May 23;5:3930
pubmed: 24852848
Plant Biotechnol J. 2016 Jan;14(1):8-21
pubmed: 26079154
Cell Biol Int. 2019 Sep;43(9):1031-1039
pubmed: 29024189
Mol Phylogenet Evol. 2010 Mar;54(3):1035-9
pubmed: 19761856
Am J Bot. 2004 Nov;91(11):1846-62
pubmed: 21652332
Electrophoresis. 2012 Sep;33(18):2840-51
pubmed: 23019101
Int J Mol Sci. 2017 Nov 03;18(11):
pubmed: 29099791
Mol Plant. 2019 Jan 7;12(1):30-43
pubmed: 30472326
Food Chem. 2016 Mar 1;194:1329-36
pubmed: 26471689
Ann Bot. 2016 Jun;117(7):1175-85
pubmed: 27098088
BMC Plant Biol. 2010 Nov 19;10:255
pubmed: 21092103
Appl Plant Sci. 2014 Jul 11;2(7):
pubmed: 25202637
Curr Opin Plant Biol. 2005 Apr;8(2):135-41
pubmed: 15752992
Plant Methods. 2018 Jun 5;14:43
pubmed: 29928291
Mol Ecol Resour. 2009 May;9 Suppl s1:130-9
pubmed: 21564972
J Exp Bot. 2000 Jul;51(348):1237-42
pubmed: 10937699
Syst Biol. 2012 Dec 1;61(6):1061-7
pubmed: 22780991
Proc Biol Sci. 2003 Feb 7;270(1512):313-21
pubmed: 12614582
Appl Plant Sci. 2017 Oct 20;5(10):
pubmed: 29109919
Front Plant Sci. 2017 Apr 20;8:604
pubmed: 28473842
Ann Bot. 2013 Jan;111(1):113-26
pubmed: 23131301
Theor Appl Genet. 2012 Mar;124(5):809-19
pubmed: 22083355
Int J Mol Sci. 2015 Sep 15;16(9):22258-79
pubmed: 26389887