Clinical Significance of Serum Galactose-Deficient IgA1 Level in Children with IgA Nephropathy.
Adolescent
Age Factors
Biomarkers
/ blood
Child
Child, Preschool
Diagnosis, Differential
Enzyme-Linked Immunosorbent Assay
Female
Galactose
/ immunology
Galactosemias
/ diagnosis
Glomerulonephritis, IGA
/ diagnosis
Humans
Immunoglobulin A
/ blood
Male
Predictive Value of Tests
Prognosis
Reference Standards
Journal
Journal of immunology research
ISSN: 2314-7156
Titre abrégé: J Immunol Res
Pays: Egypt
ID NLM: 101627166
Informations de publication
Date de publication:
2020
2020
Historique:
received:
10
12
2019
revised:
13
03
2020
accepted:
01
04
2020
entrez:
16
6
2020
pubmed:
17
6
2020
medline:
9
3
2021
Statut:
epublish
Résumé
This study was aimed at investigating the clinical significance of serum galactose-deficient IgA1 (Gd-IgA1) levels measured by a novel lectin-independent enzyme-linked immunosorbent assay (ELISA) using an anti-Gd-IgA1 monoclonal antibody (KM55) as a disease-specific biomarker for IgA nephropathy (IgAN) in children. Thirty-three children with IgAN, 40 with non-IgA glomerular diseases, and 38 age-matched healthy controls (HCs) were enrolled. Serum Gd-IgA1 levels were quantified by ELISA using KM55. Results were statistically compared with clinical features and pathological findings of IgAN. Serum Gd-IgA1 levels were significantly elevated in children with IgAN compared with children with non-IgA glomerular diseases and HCs. Serum Gd-IgA1 levels in children with IgAN were positively correlated with serum total IgA levels. However, the serum Gd-IgA1/total IgA ratio (Gd-IgA1/IgA) was also significantly elevated in children with IgAN. Serum Gd-IgA1 levels in children with IgAN increased in an age-dependent manner. The cutoff value of serum Gd-IgA1 levels for differentiating IgAN from non-IgA glomerular diseases was 3236 in children < 12 years and 5284 in children ≥ 12 years, respectively. In contrast, serum Gd-IgA1/IgA was age-independent. The cutoff value of serum Gd-IgA1/IgA for differentiating IgAN from non-IgA glomerular diseases was 0.2401. Serum Gd-IgA1 levels were negatively correlated with eGFR and positively correlated with mesangial IgA deposition. In contrast, serum Gd-IgA1/IgA levels were not correlated with any clinical parameters of IgAN. In conclusion, serum Gd-IgA1 levels were significantly elevated in children with IgAN. However, those levels were age-dependent; therefore, serum Gd-IgA1 levels classified by age and/or serum Gd-IgA1/IgA might have diagnostic values in children with IgAN.
Identifiants
pubmed: 32537466
doi: 10.1155/2020/4284379
pmc: PMC7260647
doi:
Substances chimiques
Biomarkers
0
Immunoglobulin A
0
Galactose
X2RN3Q8DNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4284379Informations de copyright
Copyright © 2020 Hitoshi Irabu et al.
Déclaration de conflit d'intérêts
The authors declare that there is no conflict of interest regarding the publication of this study.
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