One-Step Duplex Droplet Digital PCR for WT1 Overexpression.
Adult
Aged
Aged, 80 and over
Case-Control Studies
DNA
/ blood
Data Accuracy
Female
Humans
Leukemia, Myeloid, Acute
/ blood
Limit of Detection
Male
Middle Aged
Neoplasm, Residual
/ blood
Proto-Oncogene Proteins c-abl
/ blood
RNA
/ blood
Real-Time Polymerase Chain Reaction
/ methods
Sensitivity and Specificity
WT1 Proteins
/ blood
Journal
The Journal of molecular diagnostics : JMD
ISSN: 1943-7811
Titre abrégé: J Mol Diagn
Pays: United States
ID NLM: 100893612
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
25
11
2019
revised:
07
05
2020
accepted:
26
05
2020
pubmed:
17
6
2020
medline:
28
8
2021
entrez:
17
6
2020
Statut:
ppublish
Résumé
With the improvement of treatment methods in acute hematology malignancies, the development of sensitive tools for minimal residual disease assessment has become a priority. The monitoring of WT1 expression level by real-time quantitative PCR has been a standard for minimal residual disease evaluation in acute myeloid leukemia and, since 2009, has been optimized through a European LeukemiaNet effort in an established protocol with well-defined clinical end points. Building on the work of the European LeukemiaNet, this article reports the development of a novel, one-step duplex WT1/ABL1 droplet digital assay for WT1 overexpression detection. This assay provides accurate data with high precision and linearity, even at low-template concentration, while retaining strong correlation with the standardized method and therefore maintaining the framework to analyze the results in the context of acute myeloid leukemia patients.
Identifiants
pubmed: 32540368
pii: S1525-1578(20)30355-X
doi: 10.1016/j.jmoldx.2020.05.010
pii:
doi:
Substances chimiques
WT1 Proteins
0
WT1 protein, human
0
RNA
63231-63-0
DNA
9007-49-2
ABL1 protein, human
EC 2.7.10.2
Proto-Oncogene Proteins c-abl
EC 2.7.10.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1008-1019Informations de copyright
Copyright © 2020 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.