Generation of a homozygous ARX nuclear CFP (ARX
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
01
04
2020
revised:
26
05
2020
accepted:
03
06
2020
pubmed:
17
6
2020
medline:
22
6
2021
entrez:
17
6
2020
Statut:
ppublish
Résumé
The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3' fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARX
Identifiants
pubmed: 32544857
pii: S1873-5061(20)30175-6
doi: 10.1016/j.scr.2020.101874
pii:
doi:
Substances chimiques
ARX protein, human
0
Cyan Fluorescent Protein
0
Homeodomain Proteins
0
Transcription Factors
0
Green Fluorescent Proteins
147336-22-9
Glucagon
9007-92-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101874Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.