Butylated hydroxytoluene protects bull sperm surface protein-P25b in different extenders following cryopreservation.
P25b
Western blotting
bull sperm
butylated hydroxytoluene
extender
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
06
08
2019
accepted:
24
02
2020
entrez:
18
6
2020
pubmed:
1
4
2019
medline:
1
4
2019
Statut:
ppublish
Résumé
The aim of this study was to investigate the effects of different concentration of butylated hydroxytoluene (BHT) on sperm membrane surface protein "P25b" from cryopreserved bull semen in either lecithin based Bioxcell Forty-five semen samples, 15 each were extended with either BX, TEY, or CEY extender which contained different concentrations (0.0 - control, 0.5, 1.0, 1.5, 2.0, and 3.0 mM/mL) of BHT. The extended semen samples were frozen at a concentration of 20×10 Results showed that supplementation of BHT improved (p<0.05) quantity of protein P25b at concentrations of 0.5mM/mL for BX and at 1.0 mM/mL for TEY and CE when compared with the controls and other treatments. BHT supplementation at 0.5 in BX and 1.0 mM/mL in TEY and CEY has protected bull sperm fertility marker protein P25b in frozen-thawed bull sperm.
Identifiants
pubmed: 32546907
doi: 10.14202/vetworld.2020.649-654
pii: Vetworld-13-649
pmc: PMC7245718
doi:
Types de publication
Journal Article
Langues
eng
Pagination
649-654Informations de copyright
Copyright: © Khumran, et al.
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